Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1989-10-26
pubmed:databankReference
pubmed:abstractText
Linear vectors based on plasmids pGKL1 and pGKL2 from Kluyveromyces lactis were obtained by in vivo recombination in Saccharomyces cerevisiae and selected for integration of the nuclear LEU2 gene. The linear hybrid molecules obtained had no proteins attached to their 5' ends, as is found for native pGKL plasmids. However, telomere-specific sequences were added to the ends of pGKL1. In contrast to the cytoplasmically localized pGKL plasmids, the newly obtained linear hybrid vectors probably replicate within the nucleus and provide evidence that the nuclear LEU2 gene cannot be expressed in the cytoplasm.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-220264, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-232210, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-2463488, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-2536898, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-2985470, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-3029695, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-3041369, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-3138657, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-3241621, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-3319189, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-3333304, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-3344219, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-3412888, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-3449223, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-347451, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-3555493, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-3684586, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-3697067, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-375221, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-3758030, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-3763395, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-388356, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-3907856, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-4066615, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-6246368, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-6257636, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-6276088, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-6286143, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-6295879, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-6330571, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-6377091, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-6379603, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-6387625, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-6473099, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-6547529, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-6841315, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-6987527, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-7016841, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-7045080, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-7309684, http://linkedlifedata.com/resource/pubmed/commentcorrection/2779572-881736
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3931-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
In vivo construction of linear vectors based on killer plasmids from Kluyveromyces lactis: selection of a nuclear gene results in attachment of telomeres.
pubmed:affiliation
Lehrstuhl für Allgemeine Botanik, Ruhr-Universität Bochum, Federal Republic of Germany.
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