| pubmed-article:2719857 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2719857 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:2719857 | lifeskim:mentions | umls-concept:C0006556 | lld:lifeskim |
| pubmed-article:2719857 | lifeskim:mentions | umls-concept:C0028953 | lld:lifeskim |
| pubmed-article:2719857 | lifeskim:mentions | umls-concept:C0439962 | lld:lifeskim |
| pubmed-article:2719857 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:2719857 | lifeskim:mentions | umls-concept:C0369355 | lld:lifeskim |
| pubmed-article:2719857 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:2719857 | lifeskim:mentions | umls-concept:C1521871 | lld:lifeskim |
| pubmed-article:2719857 | lifeskim:mentions | umls-concept:C0205430 | lld:lifeskim |
| pubmed-article:2719857 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:2719857 | lifeskim:mentions | umls-concept:C0007512 | lld:lifeskim |
| pubmed-article:2719857 | lifeskim:mentions | umls-concept:C0206415 | lld:lifeskim |
| pubmed-article:2719857 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2719857 | pubmed:dateCreated | 1989-7-3 | lld:pubmed |
| pubmed-article:2719857 | pubmed:abstractText | Hexokinase (EC 2.7.1.1) catalyzes the first step in glucose metabolism, using ATP for the phosphorylation of glucose to glucose 6-phosphate. A portion of the HK1 gene was cloned by mixed oligonucleotide primer amplification of cDNA using primers of high complexity. The amino acid sequence for a partial fragment of bovine cardiac muscle HK was determined and used to create primer mixtures of 256- and 1024-fold complexity. Two products were generated from bovine cardiac muscle cDNA which show 82% nucleotide and 93% amino acid identity with a region of rat brain HK1 and cDNA. This work demonstrates that extension and amplification of cDNA probes may be successful even when amino acid sequence data indicate substantial codon degeneracy. | lld:pubmed |
| pubmed-article:2719857 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2719857 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2719857 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2719857 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2719857 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2719857 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2719857 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2719857 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2719857 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2719857 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:2719857 | pubmed:issn | 0885-4505 | lld:pubmed |
| pubmed-article:2719857 | pubmed:author | pubmed-author:McCabeE RER | lld:pubmed |
| pubmed-article:2719857 | pubmed:author | pubmed-author:CookR GRG | lld:pubmed |
| pubmed-article:2719857 | pubmed:author | pubmed-author:HarterJJ | lld:pubmed |
| pubmed-article:2719857 | pubmed:author | pubmed-author:GriffinL DLD | lld:pubmed |
| pubmed-article:2719857 | pubmed:author | pubmed-author:MacGregorG... | lld:pubmed |
| pubmed-article:2719857 | pubmed:author | pubmed-author:MuznyD MDM | lld:pubmed |
| pubmed-article:2719857 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2719857 | pubmed:volume | 41 | lld:pubmed |
| pubmed-article:2719857 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2719857 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2719857 | pubmed:pagination | 125-31 | lld:pubmed |
| pubmed-article:2719857 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2719857 | pubmed:meshHeading | pubmed-meshheading:2719857-... | lld:pubmed |
| pubmed-article:2719857 | pubmed:meshHeading | pubmed-meshheading:2719857-... | lld:pubmed |
| pubmed-article:2719857 | pubmed:meshHeading | pubmed-meshheading:2719857-... | lld:pubmed |
| pubmed-article:2719857 | pubmed:meshHeading | pubmed-meshheading:2719857-... | lld:pubmed |
| pubmed-article:2719857 | pubmed:meshHeading | pubmed-meshheading:2719857-... | lld:pubmed |
| pubmed-article:2719857 | pubmed:meshHeading | pubmed-meshheading:2719857-... | lld:pubmed |
| pubmed-article:2719857 | pubmed:meshHeading | pubmed-meshheading:2719857-... | lld:pubmed |
| pubmed-article:2719857 | pubmed:meshHeading | pubmed-meshheading:2719857-... | lld:pubmed |
| pubmed-article:2719857 | pubmed:meshHeading | pubmed-meshheading:2719857-... | lld:pubmed |
| pubmed-article:2719857 | pubmed:meshHeading | pubmed-meshheading:2719857-... | lld:pubmed |
| pubmed-article:2719857 | pubmed:meshHeading | pubmed-meshheading:2719857-... | lld:pubmed |
| pubmed-article:2719857 | pubmed:meshHeading | pubmed-meshheading:2719857-... | lld:pubmed |
| pubmed-article:2719857 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2719857 | pubmed:articleTitle | Synthesis and characterization of a bovine hexokinase 1 cDNA probe by mixed oligonucleotide primed amplification of cDNA using high complexity primer mixtures. | lld:pubmed |
| pubmed-article:2719857 | pubmed:affiliation | Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030. | lld:pubmed |
| pubmed-article:2719857 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2719857 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2719857 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:280817 | entrezgene:pubmed | pubmed-article:2719857 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2719857 | lld:pubmed |
