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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1989-8-23
|
| pubmed:databankReference | |
| pubmed:abstractText |
A new human homeo box-containing gene designated Hox-5.2 was cloned and mapped to human chromosome 2. This homeo box is related in sequence to Abdominal-B, a Drosophila homeotic gene that specifies identity of posterior segments. An antibody probe was made using a human Hox-5.2 fusion protein and was found to stain posterior regions of mouse, chicken, and Xenopus embryos. Unexpectedly, when the distribution of Hox-5.2 antigen was compared with that of X1Hbox 1 antigen, a non-overlapping and mutually exclusive pattern was detected (e.g., in developing limb buds, intestine, and somites). Regions expressing Hox-5.2 do not express X1Hbox 1 protein, and vice versa. Hox-5.2 antigen is detected strongly in developing fore- and hindlimb buds, where it forms a gradient of nuclear protein throughout most of the mesenchyme. This gradient is maximal in distal and posterior regions. Hox-5.2 expression is activated in Xenopus limb regeneration blastemas, as expected for any gene involved in pattern formation. As described previously, a gradient of X1Hbox 1 protein can be detected in the forelimb. The latter gradient has the opposite polarity to that of Hox-5.2. i.e., maximal in anterior and proximal mesoderm. These two opposing gradients (and possibly others) could be involved in determining positional values in developing limb buds.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0890-9369
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
3
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
641-50
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2568311-Amino Acid Sequence,
pubmed-meshheading:2568311-Animals,
pubmed-meshheading:2568311-Base Sequence,
pubmed-meshheading:2568311-Chick Embryo,
pubmed-meshheading:2568311-Chromosome Mapping,
pubmed-meshheading:2568311-Chromosomes, Human, Pair 2,
pubmed-meshheading:2568311-Cloning, Molecular,
pubmed-meshheading:2568311-Drosophila,
pubmed-meshheading:2568311-Extremities,
pubmed-meshheading:2568311-Genes, Homeobox,
pubmed-meshheading:2568311-Humans,
pubmed-meshheading:2568311-Immunochemistry,
pubmed-meshheading:2568311-Mice,
pubmed-meshheading:2568311-Mice, Inbred BALB C,
pubmed-meshheading:2568311-Molecular Sequence Data,
pubmed-meshheading:2568311-Multigene Family,
pubmed-meshheading:2568311-Nucleic Acid Hybridization,
pubmed-meshheading:2568311-Recombinant Fusion Proteins,
pubmed-meshheading:2568311-Regeneration,
pubmed-meshheading:2568311-Xenopus,
pubmed-meshheading:2568311-beta-Galactosidase
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Complementary homeo protein gradients in developing limb buds.
|
| pubmed:affiliation |
Department of Biological Chemistry, University of California, School of Medicine, Los Angeles 90024-1737.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|