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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1989-6-23
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pubmed:abstractText |
All Legionella species express a 60-kilodalton (kDa) protein which contains a genus-specific epitope recognized by murine monoclonal antibody GW2X4B8B2H6. A genomic cosmid library of Legionella pneumophila chromosomal DNA was constructed in pHC79 and screened for 60-kDa antigen-expressing clones with the monoclonal antibody. A 3.2-kilobase EcoRI fragment from cosmid 14B11 expressing a 60-kDa protein was subcloned into pUC19 (pSH16), and deletion of a 1.2-kilobase HindIII fragment (pSH16A) generated a 33-kDa truncated polypeptide no longer reactive with the monoclonal antibody. Southern blot analysis of chromosomal DNA from selected Legionella species restricted with EcoRI and probed with the 1.2-kilobase fragment coding for the carboxyl region of the protein revealed DNA homology which was not observed with DNA from Escherichia coli. Maxicell analysis of pSH16 identified a second polypeptide of approximately 15 kDa expressed from a gene (htpA) upstream of the gene coding the 60-kDa protein (htpB). Both proteins were preferentially synthesized by L. pneumophila following heat shock (temperature shift from 25 to 42 degrees C), and under steady-state growth conditions the relative level of 60-kDa protein was unaffected by temperature. In E. coli, expression of a 60-kDa protein from pSH16 also increased following heat shock (25 to 42 degrees C), but under steady-state conditions expression was temperature dependent. Temperature-dependent expression from pSH16 was not observed in an rpoH (htpR) mutant strain of E. coli. The Legionella 60-kDa protein appears to be a heat shock protein which shares cross-reactive epitopes with the GroEL homolog of E. coli. In addition, a region of htpB encoding the 27-kDa carboxyl portion of the protein containing the monoclonal antibody-reactive epitope also contains DNA sequences unique to and conserved within the genus.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-1101033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-2448638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-2892795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-2897629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-2904297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-2987191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-3029018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-3093580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-3112578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-3114149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-3132709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-3282178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-3282179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-3308708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-3315851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-3343219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-3510397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-3517046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-3623700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-3819647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-3924087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-4113792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-5432063,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-6501409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-6619736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-6644240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-7299350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2566581-7391615
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
57
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1731-9
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2566581-Antigens, Bacterial,
pubmed-meshheading:2566581-Bacterial Proteins,
pubmed-meshheading:2566581-Blotting, Southern,
pubmed-meshheading:2566581-Chaperonin 60,
pubmed-meshheading:2566581-Cloning, Molecular,
pubmed-meshheading:2566581-Cosmids,
pubmed-meshheading:2566581-Escherichia coli,
pubmed-meshheading:2566581-Genes, Bacterial,
pubmed-meshheading:2566581-Heat-Shock Proteins,
pubmed-meshheading:2566581-Legionella,
pubmed-meshheading:2566581-Molecular Weight,
pubmed-meshheading:2566581-Nucleic Acid Hybridization,
pubmed-meshheading:2566581-Restriction Mapping,
pubmed-meshheading:2566581-Temperature
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pubmed:year |
1989
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pubmed:articleTitle |
Cloning and temperature-dependent expression in Escherichia coli of a Legionella pneumophila gene coding for a genus-common 60-kilodalton antigen.
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pubmed:affiliation |
Department of Microbiology and Immunology, University of Tennessee, Memphis 38163.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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