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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1989-6-2
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pubmed:abstractText |
The binding kinetics, pharmacologic properties, ontogeny and localization of L-glutamate binding sites were studied in membrane preparations and sections of normal and olivopontocerebellar atrophy (OPCA) human cerebellum. One binding component was found with a Kd value in the order of 150 x 10(-9) M. No significant changes of Kd values were observed with age, whereas the highest Bmax value was observed at the age of 1 year. L-Aspartate, ibotenate, quisqualate and L-homocysteic acid were potent inhibitors of L-[3H]glutamate binding. Quantitative densitometric measurements indicated the presence of L-glutamate sites in both the molecular and granule cell layer. In OPCA cerebella a very significant decrease of L-[3H]glutamate specific binding (Bmax) was observed, whereas Kd values were found unchanged. The pharmacologic properties of L-[3H]glutamate binding sites of OPCA cerebellar tissues were similar to those of normal cerebellum. [3H]quinuclidinyl benzylate binding, expressed in fmol/mg protein, did not show significant differences between normal and OPCA cerebella.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-8993
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
481
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
87-96
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2565135-Adolescent,
pubmed-meshheading:2565135-Adult,
pubmed-meshheading:2565135-Aged,
pubmed-meshheading:2565135-Aged, 80 and over,
pubmed-meshheading:2565135-Aging,
pubmed-meshheading:2565135-Binding, Competitive,
pubmed-meshheading:2565135-Cerebellum,
pubmed-meshheading:2565135-Child,
pubmed-meshheading:2565135-Child, Preschool,
pubmed-meshheading:2565135-Glutamates,
pubmed-meshheading:2565135-Glutamic Acid,
pubmed-meshheading:2565135-Humans,
pubmed-meshheading:2565135-Infant,
pubmed-meshheading:2565135-Infant, Newborn,
pubmed-meshheading:2565135-Kinetics,
pubmed-meshheading:2565135-Middle Aged,
pubmed-meshheading:2565135-Oxadiazoles,
pubmed-meshheading:2565135-Quisqualic Acid,
pubmed-meshheading:2565135-Receptors, Glutamate,
pubmed-meshheading:2565135-Receptors, Neurotransmitter
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pubmed:year |
1989
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pubmed:articleTitle |
L-glutamate binding sites of normal and atrophic human cerebellum.
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pubmed:affiliation |
Department of Physiology, School of Medicine, University of Patras, Greece.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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