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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1989-7-21
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pubmed:abstractText |
Aberrations in nuclear proto-oncogene organisation and/or gene expression have been implicated in cell transformation mediated by the v-abl gene. For example, it has been suggested that amplification of the c-myc proto-oncogene is a co-operative event in v-abl induced fibroblast transformation. We have investigated amplification of the c-myc, p53 and c-fos nuclear proto-oncogenes in several Abelson murine leukaemia virus (A-MuLV) transformed fibroblast lines. None of these proto-oncogenes were detectably rearranged or amplified in v-abl transformed Swiss 3T3 lines. In contrast, NIH3T3 fibroblasts transformed by the v-abl gene consistently showed a 4 to 16-fold amplification of the c-myc gene. These data show that c-myc gene amplification is not an obligatory event associated with A-MuLV transformation, but may be restricted to cell lines derived from NIH3T3. c-myc gene amplification also did not correlate with a reduced latency period for tumour induction in nude mice. In addition, c-myc amplification was not selected during tumourigenesis, indicating that this event is not required for A-MuLV transformed Swiss 3T3 cells to display a full tumourigenic phenotype.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
|
pubmed:issn |
0950-9232
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
4
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
753-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2543944-Abelson murine leukemia virus,
pubmed-meshheading:2543944-Animals,
pubmed-meshheading:2543944-Blotting, Southern,
pubmed-meshheading:2543944-Cell Line, Transformed,
pubmed-meshheading:2543944-Fibroblasts,
pubmed-meshheading:2543944-Gene Amplification,
pubmed-meshheading:2543944-Leukemia Virus, Murine,
pubmed-meshheading:2543944-Proto-Oncogenes,
pubmed-meshheading:2543944-RNA, Messenger
|
pubmed:year |
1989
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pubmed:articleTitle |
Analysis of A-MuLV transformed fibroblast lines for amplification of the c-myc, p53 and c-fos nuclear proto-oncogenes.
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pubmed:affiliation |
Laboratory of Eukaryotic Molecular Genetics, National Institute for Medical Research, Mill Hill, London.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|