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rdf:type |
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lifeskim:mentions |
umls-concept:C0025255,
umls-concept:C0030685,
umls-concept:C0391871,
umls-concept:C0597427,
umls-concept:C0680255,
umls-concept:C0728938,
umls-concept:C1283071,
umls-concept:C1320928,
umls-concept:C1549439,
umls-concept:C1749467,
umls-concept:C1963578,
umls-concept:C2347958,
umls-concept:C2717940
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pubmed:issue |
1
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pubmed:dateCreated |
1989-4-6
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pubmed:abstractText |
Secretory proteins migrate from the rough endoplasmic reticulum (ER) to the Golgi complex at different rates. Selective retention of specific proteins to rough-ER membrane constituents could explain this phenomenon. We have permeabilized HepG2 cells with low concentrations of saponin. Release of newly synthesized proteins was studied after brief labelling in the presence of [35S]methionine. The efflux of several secretory proteins was studied at various saponin concentrations; a 2-fold higher saponin concentration was required to release transferrin compared with that required to release albumin and orosomucoid. Glucosidase II, a soluble resident protein of the ER, is released at the same saponin concentration as albumin. Saponin did not destroy the membrane skeleton structure; at the concentrations used, the integral membrane protein G of vesicular-stomatitis virus remained fully associated with the cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-10605441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-13966357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-2871556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-2954588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-3292055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-3304148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-3402439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-3426543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-3545499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-3546312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-3594573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-4391354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-5134192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-5479418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-6257395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-6324191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-6373255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-6538481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-6866094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-7142193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537621-7354212
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
257
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
159-63
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:2537621-Albumins,
pubmed-meshheading:2537621-Carcinoma, Hepatocellular,
pubmed-meshheading:2537621-Cell Line,
pubmed-meshheading:2537621-Endoplasmic Reticulum,
pubmed-meshheading:2537621-Humans,
pubmed-meshheading:2537621-Intracellular Membranes,
pubmed-meshheading:2537621-Liver Neoplasms,
pubmed-meshheading:2537621-Neoplasm Proteins,
pubmed-meshheading:2537621-Permeability,
pubmed-meshheading:2537621-Saponins,
pubmed-meshheading:2537621-Solubility,
pubmed-meshheading:2537621-Transferrin,
pubmed-meshheading:2537621-Tumor Cells, Cultured
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pubmed:year |
1989
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pubmed:articleTitle |
Release of soluble resident as well as secretory proteins from HepG2 cells by partial permeabilization of rough-endoplasmic-reticulum membranes.
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pubmed:affiliation |
Laboratory of Cell Biology, University of Utrecht, Medical School, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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