| pubmed-article:2521355 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2521355 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2521355 | lifeskim:mentions | umls-concept:C0229664 | lld:lifeskim |
| pubmed-article:2521355 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:2521355 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:2521355 | lifeskim:mentions | umls-concept:C0087050 | lld:lifeskim |
| pubmed-article:2521355 | lifeskim:mentions | umls-concept:C1823153 | lld:lifeskim |
| pubmed-article:2521355 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:2521355 | lifeskim:mentions | umls-concept:C2349976 | lld:lifeskim |
| pubmed-article:2521355 | lifeskim:mentions | umls-concept:C1552644 | lld:lifeskim |
| pubmed-article:2521355 | lifeskim:mentions | umls-concept:C1880371 | lld:lifeskim |
| pubmed-article:2521355 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2521355 | pubmed:dateCreated | 1989-2-21 | lld:pubmed |
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| pubmed-article:2521355 | pubmed:abstractText | We have investigated the diversity and repertoire of human TCR delta chain variable gene segments in the human peripheral blood CD4- CD8- (double-negative) population, using rearrangement and expression studies and sequence analyses. 20 TCR delta DNA clones were derived from the RNA of bulk-cultured double-negative T cells and their nucleotide sequences determined. These clones can be classified into six different V delta subfamilies. The distribution, however, was uneven in these cells, with 16 of 20 being derived from the V delta 1 (9) and V delta 2 (7) subfamilies. The remaining subfamilies, V delta 3, V delta 4, V delta 5, and V delta 6, were only represented by one clone each. The majority of these subfamilies seem to consist of a single member, in contrast with the closely linked V alpha subfamilies, which, in most cases, consist of multiple members. Our findings suggest that only a limited number of V delta genes are used in human peripheral blood double-negative T cells and that two major V delta subfamilies (V delta 1 and V delta 2) are used more frequently. Sequence comparison of our cDNA clones to V alpha clones indicates that there is no overlap in usage of V alpha and V delta gene segments, except for the V delta 4 (V alpha 6) subfamily. Comparison of the different V delta sequences suggests that the majority of the sequence diversity is concentrated in the junctions between V, D, and J segments and results from extensive N region diversity. | lld:pubmed |
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| pubmed-article:2521355 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2521355 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2521355 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2521355 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2521355 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2521355 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:2521355 | pubmed:issn | 0022-1007 | lld:pubmed |
| pubmed-article:2521355 | pubmed:author | pubmed-author:ReimannJJ | lld:pubmed |
| pubmed-article:2521355 | pubmed:author | pubmed-author:MaoT STS | lld:pubmed |
| pubmed-article:2521355 | pubmed:author | pubmed-author:MorettaLL | lld:pubmed |
| pubmed-article:2521355 | pubmed:author | pubmed-author:CicconeEE | lld:pubmed |
| pubmed-article:2521355 | pubmed:author | pubmed-author:TakiharaYY | lld:pubmed |
| pubmed-article:2521355 | pubmed:author | pubmed-author:Michalopoulos... | lld:pubmed |
| pubmed-article:2521355 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2521355 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:2521355 | pubmed:volume | 169 | lld:pubmed |
