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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0017262,
umls-concept:C0021920,
umls-concept:C0026544,
umls-concept:C0032131,
umls-concept:C0035820,
umls-concept:C0086222,
umls-concept:C0205360,
umls-concept:C0430991,
umls-concept:C0740302,
umls-concept:C1158478,
umls-concept:C1257994,
umls-concept:C1366332,
umls-concept:C1366516,
umls-concept:C1705422,
umls-concept:C1705733,
umls-concept:C1706474,
umls-concept:C2603363
|
| pubmed:issue |
35
|
| pubmed:dateCreated |
1990-1-19
|
| pubmed:abstractText |
Rearranged MOPC41 immunoglobulin kappa gene constructs have been stably introduced into cultured S194 mouse plasmacytoma cells to investigate the effects of deleting the intronic enhancer and/or matrix association region (MAR) on gene expression. Intact single-copy kappa genes containing 1.5 kilobase pairs of upstream and 8.5 kilobase pairs of downstream flanking sequences exhibited sensitivity to chromosome position effects and were expressed at a mean level of 27% relative to the endogenous kappa gene expression or only 6% with respect to the MOPC41 kappa mRNA levels in the tumor. Deletion of the intronic MAR led to a 4-fold decrease in expression, while deletion of both the MAR and enhancer led to an 11-fold decline. These effects were dampened by preselecting for integration into a transcriptionally poised chromatin location as demonstrated by linkage to a selectable marker which lacked both a MAR and an enhancer. Significantly, we found that sequences downstream of the poly(A) addition site compensated 150-fold for deletion of the intronic enhancer.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
264
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
21183-9
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:2512290-Animals,
pubmed-meshheading:2512290-Blotting, Northern,
pubmed-meshheading:2512290-Blotting, Southern,
pubmed-meshheading:2512290-Cell Line,
pubmed-meshheading:2512290-DNA, Neoplasm,
pubmed-meshheading:2512290-Enhancer Elements, Genetic,
pubmed-meshheading:2512290-Gene Expression,
pubmed-meshheading:2512290-Genes, Immunoglobulin,
pubmed-meshheading:2512290-Immunoglobulin kappa-Chains,
pubmed-meshheading:2512290-Introns,
pubmed-meshheading:2512290-Mice,
pubmed-meshheading:2512290-Plasmacytoma,
pubmed-meshheading:2512290-Plasmids,
pubmed-meshheading:2512290-Restriction Mapping,
pubmed-meshheading:2512290-Transfection
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Immunoglobulin kappa gene expression after stable integration. I. Role of the intronic MAR and enhancer in plasmacytoma cells.
|
| pubmed:affiliation |
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|