Linked Life Data

2511069

Source:http://linkedlifedata.com/resource/pubmed/id/2511069

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-1-5
pubmed:abstractText
The genetic organization of interval 62B3-4 to 62D3-4 on the Drosophila third chromosome was investigated. The region (designated DRE) includes four known loci: Roughened (R; 3-1.4), defined by a dominant mutation disrupting eye morphology; the nonvital locus Aprt, structural gene for adenine phosphoribosyltransferase; Dras3, a homolog of the vertebrate ras oncogene; and 1(3)ecdysoneless (1(3)ecd), a gene that has been implicated in the regulation of larval molting hormone (ecdysteroid) synthesis. Overlapping chromosomal deletions of the region were generated by gamma-ray-induced reversion of the R mutation. Recessive lethal mutations were isolated based upon failure to complement the recessive lethality of Df(3L)RR2, a deletion of the DRE region that removes 16-18 polytene chromosome bands. A total of 117 mutations were isolated following ethyl methanesulfonate and gamma-ray mutagenesis. These and two additional define 13 lethal complementation groups. Mutations at two loci were recovered at disproportionately high rates. One of these loci is preferentially sensitive to radiation-induced mutational alterations. Additionally, an unusually low recovery rate for cytologically detectable rearrangement breakpoints within the gamma-ray-sensitive locus suggests that an interval of the DRE region closely linked to the R locus may be dominantly sensitive to position effects. Lethal phase analysis of mutant hemizygotes indicates that a high proportion of DRE-region loci (11 of 13) are necessary for larval development. Mutations in five loci cause predominantly first-instar larval lethality, while mutations in four other loci cause predominantly second-instar lethality. Mutations in two loci cause late-larval lethality associated with abnormal imaginal disc development. A temperature-sensitive allele of one newly identified complementation group blocks ecdysteroid-induced pupariation. This developmental block is overcome by dietary 20-hydroxyecdysone, suggesting that a second locus in the region in addition to l(3)ecd may play a role in the regulation of late larval ecdysteroid levels.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511069-17246168, http://linkedlifedata.com/resource/pubmed/commentcorrection/2511069-17246190, http://linkedlifedata.com/resource/pubmed/commentcorrection/2511069-2512110, http://linkedlifedata.com/resource/pubmed/commentcorrection/2511069-2830671, http://linkedlifedata.com/resource/pubmed/commentcorrection/2511069-2996782, http://linkedlifedata.com/resource/pubmed/commentcorrection/2511069-3091446, http://linkedlifedata.com/resource/pubmed/commentcorrection/2511069-3097510, http://linkedlifedata.com/resource/pubmed/commentcorrection/2511069-3102296, http://linkedlifedata.com/resource/pubmed/commentcorrection/2511069-3125085, http://linkedlifedata.com/resource/pubmed/commentcorrection/2511069-4624778, http://linkedlifedata.com/resource/pubmed/commentcorrection/2511069-6056758, http://linkedlifedata.com/resource/pubmed/commentcorrection/2511069-6430564, http://linkedlifedata.com/resource/pubmed/commentcorrection/2511069-6778775, http://linkedlifedata.com/resource/pubmed/commentcorrection/2511069-6806144, http://linkedlifedata.com/resource/pubmed/commentcorrection/2511069-811500
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0016-6731
pubmed:author
pubmed:issnType
Print
pubmed:volume
123
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
327-36
pubmed:dateRevised
2010-9-10
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
The genetics of the Dras3-Roughened-ecdysoneless chromosomal region (62B3-4 to 62D3-4) in Drosophila melanogaster: analysis of recessive lethal mutations.
pubmed:affiliation
Department of Biology, University of North Carolina, Chapel Hill 27599-3280.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.