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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1989-6-30
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pubmed:abstractText |
1. Protein constituents were determined in eight amyloid deposits from eight patients (five male and three female), 53 +/- 4 years of age, treated by haemodialysis for 9-20 years using only cuprophane membranes and operated for carpal tunnel syndrome. 2. Soluble proteins were removed by solubilization in phosphate-buffered saline after osmotic lysis. The proteins of the insoluble fibrils were characterized by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and two-dimensional gel electrophoresis, and immunologically identified by Western blotting. 3. In addition to beta 2-microglobulin, alpha 2-macroglobulin was identified in the fibrillar material. The presence of these two proteins in amyloid deposits was confirmed by immunofluorescent microscopic studies. 4. Our data confirm the presence of beta 2-microglobulin in haemodialysis-associated amyloidosis, and also suggest a possible role for alpha 2-microglobulin: it may protect beta 2-microglobulin from proteolytic digestion, leading to its accumulation in intact form and to amyloid fibril formation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0143-5221
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
76
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
547-52
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:2470544-Amyloid,
pubmed-meshheading:2470544-Amyloidosis,
pubmed-meshheading:2470544-Blotting, Western,
pubmed-meshheading:2470544-Carpal Tunnel Syndrome,
pubmed-meshheading:2470544-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2470544-Humans,
pubmed-meshheading:2470544-Middle Aged,
pubmed-meshheading:2470544-Molecular Weight,
pubmed-meshheading:2470544-Proteins,
pubmed-meshheading:2470544-Renal Dialysis,
pubmed-meshheading:2470544-alpha-Macroglobulins,
pubmed-meshheading:2470544-beta 2-Microglobulin
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pubmed:year |
1989
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pubmed:articleTitle |
High-molecular-mass proteins in haemodialysis-associated amyloidosis.
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pubmed:affiliation |
INSERM Unit 249, Centre de Recherche Biochimie Macromoléculaire, CNRS, Montpellier, France.
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pubmed:publicationType |
Journal Article
|