Linked Life Data

2461671

Source:http://linkedlifedata.com/resource/pubmed/id/2461671

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1989-1-12
pubmed:abstractText
Leukotriene B4 (LTB4) is a potent stimulus for neutrophil chemotaxis, aggregation, and activation. Although this mediator has been postulated as a possible stimulus for the neutrophil accumulation seen after mast cell triggering in vivo, the ability of human mast cells to produce this leukotriene has never been described. The purpose of this study was to investigate the ability of purified human lung mast cells and mast cells in lung fragments to generate leukotriene B4 after immunologic (anti-IgE) and nonimmunologic (calcium ionophore, A23187) activation. Release of LTB4 was quantitated by 2 specific radioimmunoassays with biochemical characterization by high performance liquid chromatography. In a first series of experiments using radioimmunoassay 1, purified human lung mast cells (n = 10) released LTB4 in response to both an immunologic and nonimmunologic stimulus in a time- and dose-dependent manner. In response to an optimum concentration of anti-IgE (3 micrograms/ml), mast cells of 4.5 to 98% purity, generated 6.2 +/- 1.7 ng immunoreactive LTB4 (iLTB4)/10(6) mast cells. HPLC characterization revealed that 30 +/- 0.3% of the iLTB4 coeluted with standard synthetic LTB4 in these studies. In a second series of experiments using radioimmunoassay 2, mast cell activation resulted in the release of 0.5 to 1 ng iLTB4/10(6) mast cells with greater than 75% coeluting with synthetic LTB4. Thus, we estimate that human lung mast cells can generate approximately 1 to 2 ng of LTB4 per million mast cells.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0003-0805
pubmed:author
pubmed:issnType
Print
pubmed:volume
138
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
389-94
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Generation of leukotriene B4 by human lung fragments and purified human lung mast cells.
pubmed:affiliation
Department of Medicine, Johns Hopkins University School of Medicine, Good Samaritan Hospital, Baltimore, Maryland.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.