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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
1986-1-6
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pubmed:abstractText |
Two site-specific antibodies that immunoprecipitate the human insulin receptor have been prepared by immunizing rabbits with chemically synthesized peptides derived from the cDNA-predicted amino acid sequence of the beta subunit of the proreceptor. Antibodies to the carboxyl terminus (AbP5) and to a domain around tyrosine-960 (AbP4) specifically recognize the beta subunit of the receptor on immunoblots. Both antibodies immunoprecipitated 125I-labeled insulin-receptor complexes and the autophosphorylated receptor. Although neither antibody inhibited insulin binding to the receptor, both insulin-dependent autophosphorylation and exogenous substrate phosphorylation were inhibited by AbP4. Inhibition by AbP4 was dependent upon the phosphorylation state of the receptor; it was not detected when the receptor was autophosphorylated prior to addition of AbP4. AbP4 did not inhibit activity of the related epidermal growth factor (EGF)-receptor tyrosine protein kinase nor did it inhibit the activity of cAMP-dependent kinase or protein kinase C. The observation that an antibody directed to residues 952-967 of the proreceptor neutralizes the protein kinase activity of the beta subunit suggests that this region may play a critical role in the function of the hormone-dependent, protein tyrosine-specific kinase activity of the insulin receptor.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-1069300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-1201912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-170678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-279910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-291908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-2983222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-429356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-4346806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6141638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6167985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6188161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6203118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6246084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6266278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6294652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6298786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6300088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6304046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-632238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6336757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6351725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6363065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6368536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6368559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6406486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6411700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6417656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6538876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6571987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6574482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-663609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6749547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6757253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6793584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6849137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-6938960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-7009609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-7021559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-7031900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-7421598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415975-908753
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7899-903
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2415975-Amino Acid Sequence,
pubmed-meshheading:2415975-Antibodies,
pubmed-meshheading:2415975-Antibody Specificity,
pubmed-meshheading:2415975-Antigen-Antibody Reactions,
pubmed-meshheading:2415975-Epitopes,
pubmed-meshheading:2415975-Humans,
pubmed-meshheading:2415975-Oligopeptides,
pubmed-meshheading:2415975-Phosphoproteins,
pubmed-meshheading:2415975-Phosphorylation,
pubmed-meshheading:2415975-Protein Precursors,
pubmed-meshheading:2415975-Protein-Tyrosine Kinases,
pubmed-meshheading:2415975-Receptor, Insulin
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pubmed:year |
1985
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pubmed:articleTitle |
An antipeptide antibody that specifically inhibits insulin receptor autophosphorylation and protein kinase activity.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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