Linked Life Data

2414038

Source:http://linkedlifedata.com/resource/pubmed/id/2414038

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1985-12-11
pubmed:abstractText
We discuss the use of "high-performance" liquid chromatography with electrochemical detection for the quantification fo serotonin (5-hydroxytryptamine) and metabolically related products (i. e., 5-hydroxyindoleacetic acid and 5-hydroxytryptophan) in biological matrices. Two methods are described: one for routine quantification of nanogram amounts or more; the other a two-column purification-separation ("trace enrichment") technique for determining subnanogram amounts of these compounds. Various factors affecting the liquid-chromatographic separation and quantification of 5-hydroxy-indoles are detailed, including selection of appropriate internal standard, sample purification, and effects of changes in mobile phase composition, e.g., ion-pair reagent, organic modifier content, and pH. We describe in detail the trace-enrichment method for determinations in the range of 25 pg to 25 ng, and discuss the general factors to consider when developing or modifying an assay for specific applications.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0009-9147
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1849-54
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Analytical considerations for quantitative determination of serotonin and its metabolically related products in biological matrices.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.