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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1990-9-11
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pubmed:abstractText |
Previous immunohistochemical work has indicated that terminal C5b-9 complement complexes are selectively deposited in infarcted areas of human myocardium. In the present study, we sought to quantify C5b-9 levels in myocardial tissue, and to differentiate between the membrane-bound C5b-9 (m) and the cytolytically inactive SC5b-9 complex. Paired tissue specimens from infarcted and non-infarcted myocardium were obtained from 36 autopsies. The homogenized and washed tissues were extracted with n-octyl-beta-D-glucopyranoside (octylglucoside) detergent, and the concentrations of C5b-9 in the extracts were determined by ELISA. Membrane-derived C5b-9 (m) and SC5b-9 were differentiated from each other on the basis of their characteristic sedimentation behaviour in sucrose density gradients. It was found that infarcted myocardial tissue contained on average an approximately three-fold higher concentration of C5b-9, compared with non-infarcted tissue. This increase was due in part to an increase in levels of C5b-9 (m). The results corroborate previous immunohistochemical data and show that complement activation occurs to completion with the generation of potentially cytotoxic C5b-9 complexes in infarcted myocardial tissues.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-227745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-2415178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-2426360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-2466593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-2981404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-3303271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-342601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-3528291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-3584995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-4035298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-4697834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-51500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-569173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-6166677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-6248769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-6272733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-6349686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-641147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-6827108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-6940190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-6974732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-7130282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2379317-808560
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
81
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
132-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2379317-Adult,
pubmed-meshheading:2379317-Aged,
pubmed-meshheading:2379317-Autopsy,
pubmed-meshheading:2379317-Complement Membrane Attack Complex,
pubmed-meshheading:2379317-Complement System Proteins,
pubmed-meshheading:2379317-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:2379317-Glycoproteins,
pubmed-meshheading:2379317-Humans,
pubmed-meshheading:2379317-Middle Aged,
pubmed-meshheading:2379317-Myocardial Infarction,
pubmed-meshheading:2379317-Myocardium
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pubmed:year |
1990
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pubmed:articleTitle |
Quantitative measurement of SC5b-9 and C5b-9(m) in infarcted areas of human myocardium.
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pubmed:affiliation |
Institute of Medical Microbiology, University of Giessen, West Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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