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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
1990-6-13
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pubmed:abstractText |
Changes in methemoglobin (Met-Hb) concentrations during storage of whole blood or mixtures of blood and a cryoprotectant at refrigerated or various freezing temperatures were examined using blood samples from nitrite-administered rats and from autopsy cadavers. When whole blood was stored at 3 degrees C, Met-Hb reduction was observed in blood samples from nitrite-administered rats and in the blood from a victim poisoned by a weed killer containing some oxidant. When samples were stored at -30 degrees C, Met-Hb formation by autoxidation was inevitably observed in blood samples stored as whole blood, whereas addition of a cryoprotectant to whole blood could prevent Met-Hb formation in all the blood samples. When whole blood was stored at -80 degrees C or -196 degrees C, Met-Hb concentrations were practically stable until at least 30 days regardless of the initial values except in the control rat blood samples stored at -80 degrees C which showed slight formation of Met-Hb. From the results obtained, both the storage with a cryoprotectant at -30 degrees C and that without any additions at -80 degrees C or -196 degrees C proved to be suitable for long-term storage of blood samples from autopsy cadavers for Met-Hb determination.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0379-0738
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
129-34
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:2335326-Animals,
pubmed-meshheading:2335326-Blood Preservation,
pubmed-meshheading:2335326-Cryopreservation,
pubmed-meshheading:2335326-Cryoprotective Agents,
pubmed-meshheading:2335326-Cytochrome-B(5) Reductase,
pubmed-meshheading:2335326-Drug Stability,
pubmed-meshheading:2335326-Hot Temperature,
pubmed-meshheading:2335326-Humans,
pubmed-meshheading:2335326-Male,
pubmed-meshheading:2335326-Methemoglobin,
pubmed-meshheading:2335326-Nitrites,
pubmed-meshheading:2335326-Rats,
pubmed-meshheading:2335326-Rats, Inbred Strains
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pubmed:year |
1990
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pubmed:articleTitle |
Storage of blood for methemoglobin determination: comparison of storage with a cryoprotectant at -30 degrees C and without any additions at -80 degrees C or -196 degrees C.
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pubmed:affiliation |
Department of Legal Medicine, Nagoya University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study
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