| pubmed-article:2307127 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2307127 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2307127 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:2307127 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:2307127 | lifeskim:mentions | umls-concept:C0070099 | lld:lifeskim |
| pubmed-article:2307127 | lifeskim:mentions | umls-concept:C0678223 | lld:lifeskim |
| pubmed-article:2307127 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:2307127 | pubmed:dateCreated | 1990-4-5 | lld:pubmed |
| pubmed-article:2307127 | pubmed:abstractText | The mechanism by which parathyroid hormone-related protein (PTH-RP) stimulates bone resorption is not known. Like certain other resorbing agents it may act to release bone-resorbing cytokines from the osteoblast. To examine this hypothesis, we used serum-free conditioned media (CM) from SAOS II cells incubated with 10(-8) M h(1-74) PTH-RP for 48 h. Treated CM contained substantially more bone-resorbing activity (BRA) in the fetal-rat long-bone assay than CM from untreated cells (2.17 +/- 0.21 vs 1.38 +/- 0.16 fold stimulation over basal [f]; p less than 0.05]. After centrifugation and dialysis, 1 liter of treated CM contained a total BRA of 7102 ngeq b(1-34) PTH with a specific activity (SA) of 447 ngeq b(1-34) PTH/mg protein. Treated CM did not stimulate the ROS assay and the cytokines PGE2, TGF-alpha, EGF, GM-CSF and IL-1 were present in low concentrations. The BRA was heat sensitive. Ultrafiltration revealed that 97% of the BRA was in a 3-30 kD fraction. Further purification was achieved by sequential reverse phase HPLC and size exclusion-HPLC (SE-HPLC). A single fraction containing BRA from SE-HPLC was purified 277-fold to a SA of 123,810 ngeq b(1-34) PTH/mg protein and had an apparent MW of 9 kD. SDS-PAGE revealed 4 bands in this SE-HPLC fraction with 1 band at 9 kD unique to that fraction. PTH-RP may cause bone resorption in part by stimulating the release of a 9 kD protein from osteoblasts which is responsible for activating osteoclasts. | lld:pubmed |
| pubmed-article:2307127 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2307127 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2307127 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2307127 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:2307127 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2307127 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2307127 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2307127 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2307127 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2307127 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2307127 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2307127 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2307127 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:2307127 | pubmed:issn | 0013-7227 | lld:pubmed |
| pubmed-article:2307127 | pubmed:author | pubmed-author:MorrisC ACA | lld:pubmed |
| pubmed-article:2307127 | pubmed:author | pubmed-author:HorowitzMM | lld:pubmed |
| pubmed-article:2307127 | pubmed:author | pubmed-author:WeirE CEC | lld:pubmed |
| pubmed-article:2307127 | pubmed:author | pubmed-author:KreiderB LBL | lld:pubmed |
| pubmed-article:2307127 | pubmed:author | pubmed-author:InsognaK LKL | lld:pubmed |
| pubmed-article:2307127 | pubmed:author | pubmed-author:MitnickM EME | lld:pubmed |
| pubmed-article:2307127 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2307127 | pubmed:volume | 126 | lld:pubmed |
| pubmed-article:2307127 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2307127 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2307127 | pubmed:pagination | 1783-5 | lld:pubmed |
| pubmed-article:2307127 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2307127 | pubmed:meshHeading | pubmed-meshheading:2307127-... | lld:pubmed |
| pubmed-article:2307127 | pubmed:meshHeading | pubmed-meshheading:2307127-... | lld:pubmed |
| pubmed-article:2307127 | pubmed:meshHeading | pubmed-meshheading:2307127-... | lld:pubmed |
| pubmed-article:2307127 | pubmed:meshHeading | pubmed-meshheading:2307127-... | lld:pubmed |
| pubmed-article:2307127 | pubmed:meshHeading | pubmed-meshheading:2307127-... | lld:pubmed |
| pubmed-article:2307127 | pubmed:meshHeading | pubmed-meshheading:2307127-... | lld:pubmed |
| pubmed-article:2307127 | pubmed:meshHeading | pubmed-meshheading:2307127-... | lld:pubmed |
| pubmed-article:2307127 | pubmed:meshHeading | pubmed-meshheading:2307127-... | lld:pubmed |
| pubmed-article:2307127 | pubmed:meshHeading | pubmed-meshheading:2307127-... | lld:pubmed |
| pubmed-article:2307127 | pubmed:meshHeading | pubmed-meshheading:2307127-... | lld:pubmed |
| pubmed-article:2307127 | pubmed:meshHeading | pubmed-meshheading:2307127-... | lld:pubmed |
| pubmed-article:2307127 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2307127 | pubmed:articleTitle | The parathyroid hormone-related protein stimulates human osteoblast-like cells to secrete a 9,000 dalton bone-resorbing protein. | lld:pubmed |
| pubmed-article:2307127 | pubmed:affiliation | Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510. | lld:pubmed |
| pubmed-article:2307127 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2307127 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2307127 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2307127 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2307127 | lld:pubmed |
