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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1990-12-5
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pubmed:abstractText |
The hematopoietic growth factor GM-CSF (granulocyte-macrophage colony-stimulating factor) is expressed by activated but not resting T lymphocytes. Previously, we localized GM-CSF-inducible promoter activity to a 90-bp region of GM-CSF 5'-flanking sequences extending from bp -53 to +37. To more precisely identify the GM-CSF DNA sequences required for inducible promoter activity in T lymphocytes, we have performed mutagenesis within a region of GM-CSF 5'-flanking sequences (bp -57 to -24) that contains the repeated sequence CATT(A/T). Mutations that do not alter the repeated CATT(A/T) sequence do not eliminate inducible promoter activity, whereas mutation or deletion of either of the CATT(A/T) repeats eliminates all inducible promoter activity in T-cell lines and in primary human T lymphocytes. Thus, both copies of the direct repeat CATT(A/T) are required for mitogen-inducible expression of GM-CSF in T cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2233734-2406568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2233734-2660069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2233734-2674282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2233734-2677143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2233734-2678102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2233734-2833332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2233734-2838738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2233734-2854202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2233734-2990035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2233734-3133208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2233734-3257571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2233734-3258537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2233734-3296190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2233734-3490669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2233734-3876931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2233734-3923623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2233734-6960240
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:geneSymbol |
hIL-5,
mIL-5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6084-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2233734-Animals,
pubmed-meshheading:2233734-Base Sequence,
pubmed-meshheading:2233734-Cell Line,
pubmed-meshheading:2233734-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:2233734-Humans,
pubmed-meshheading:2233734-Interleukin-5,
pubmed-meshheading:2233734-Mice,
pubmed-meshheading:2233734-Molecular Sequence Data,
pubmed-meshheading:2233734-Mutagenesis, Site-Directed,
pubmed-meshheading:2233734-Promoter Regions, Genetic,
pubmed-meshheading:2233734-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:2233734-Restriction Mapping,
pubmed-meshheading:2233734-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2233734-T-Lymphocytes
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pubmed:year |
1990
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pubmed:articleTitle |
The repeated sequence CATT(A/T) is required for granulocyte-macrophage colony-stimulating factor promoter activity.
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pubmed:affiliation |
Department of Medicine, University of California, Los Angeles, School of Medicine.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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