Linked Life Data

2211366

Source:http://linkedlifedata.com/resource/pubmed/id/2211366

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1990-11-21
pubmed:abstractText
We cloned DNA fragments of sisomicin-producing Micromonospora inyoensis into Streptomyces plasmid vectors and identified Streptomyces lividans TK24 transformants expressing the M. inyoensis sisomicin-resistance (sisA) gene. The sisA gene was compared to the previously reported Micromonospora purpurea Kan-Gen (kanamycin-gentamicin)-resistance gene. While the restriction endonuclease digestion patterns of the two determinants appear to be divergent, the genes are nonetheless closely related, based on similar patterns and levels of aminoglycoside-resistance and their ability to cross-hybridize under stringent conditions. We have transformed recombinant plasmid pMD5-2, which carries the sisA gene, into our M. purpurea gentamicin-production strain and determined that gentamicin biosynthesis was not improved.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-8820
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:geneSymbol
sisA
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
992-9
pubmed:dateRevised
2006-5-1
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Cloning and characterization of the sisomicin-resistance gene from Micromonospora inyoensis.
pubmed:affiliation
Department of Microbiological and Cell Culture Development, Schering Corporation, Union, NJ 07083.
pubmed:publicationType
Journal Article