Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
|
pubmed:dateCreated |
1990-5-15
|
pubmed:abstractText |
An aqueous solution of calf thymus DNA was irradiated by 60Co gamma-rays and modified nucleosides produced in DNA were analyzed by high-pressure liquid chromatography coupled with a photodiode array UV detector. A new product with UV absorption maxima at 230 nm and 280 nm was observed. The structure of this compound was proposed to be 5-formyldeoxyuridine (f5dU) based on the mass spectrum of its trimethylsilyl derivative (M+, m/z472) and the structure was confirmed by chemical synthesis. The yield of f5dU (2.4/10(4) dT/krad) in DNA was of roughly the same order as that of 8-hydroxydeoxyguanosine and 5-hydroxymethyldeoxyuridine. Free f5dU was mutagenic to Salmonella typhimurium strain TA102: therefore f5dU incorporated into DNA may induce mutations.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0027-5107
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
243
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
249-53
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:2183040-Animals,
pubmed-meshheading:2183040-Cattle,
pubmed-meshheading:2183040-Chromatography, High Pressure Liquid,
pubmed-meshheading:2183040-DNA,
pubmed-meshheading:2183040-DNA Damage,
pubmed-meshheading:2183040-Deoxyuridine,
pubmed-meshheading:2183040-Dose-Response Relationship, Radiation,
pubmed-meshheading:2183040-Gamma Rays,
pubmed-meshheading:2183040-Mass Spectrometry,
pubmed-meshheading:2183040-Molecular Structure,
pubmed-meshheading:2183040-Mutagenicity Tests,
pubmed-meshheading:2183040-Mutagens,
pubmed-meshheading:2183040-Salmonella typhimurium
|
pubmed:year |
1990
|
pubmed:articleTitle |
5-Formyldeoxyuridine: a new type of DNA damage induced by ionizing radiation and its mutagenicity to salmonella strain TA102.
|
pubmed:affiliation |
Biology Division, National Cancer Center Research Institute, Tokyo, Japan.
|
pubmed:publicationType |
Journal Article
|