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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1990-4-17
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pubmed:abstractText |
A sandwich ELISA for rat apo E was developed. Blood was drawn from rats that had been administered with triamcinolone diacetate to increase apo E-rich HDL in serum. Apo E was purified from the d less than 1.225 g/ml lipoproteins, and antiserum was raised in rabbits. Diluted samples and standards were added into the wells of polystyrene microtiter plates precoated with immunoaffinity-purified IgG and incubated for 90 min. After washing, immunoaffinity-purified Fab'-horseradish peroxidase conjugate was added to each well and incubated for 90 min. The bound enzyme was assayed by a colorimetric method. Samples and standards were pretreated with 6 M guanidine. HCl to maximize the antigenic response of apo E. The sensitivity lies around 1 pg apo E, and the working range was 0.1 to 1.0 ng. All assay procedures were completed within 4-5 hr. The mean intra- and interassay coefficients of variation were 1.8 and 4.1%, respectively. Serum apo E concentrations were 21.2 +/- 2.1 and 61.3 +/- 17.0 mg/dl (mean +/- SD) for young (8-12 weeks old, n = 9) and old (36-40 weeks old, n = 16) rats, respectively. As determined by gel filtrations, most of the apo E in fasted rat serum was associated with larger HDL particles (or HDL1) and a small portion of apo E was present in a free form.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0885-4505
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
43
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
53-64
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2178652-Aging,
pubmed-meshheading:2178652-Animals,
pubmed-meshheading:2178652-Apolipoproteins E,
pubmed-meshheading:2178652-Body Weight,
pubmed-meshheading:2178652-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:2178652-Immunoenzyme Techniques,
pubmed-meshheading:2178652-Immunoglobulin G,
pubmed-meshheading:2178652-Lipids,
pubmed-meshheading:2178652-Male,
pubmed-meshheading:2178652-Rabbits,
pubmed-meshheading:2178652-Radioimmunoassay,
pubmed-meshheading:2178652-Rats,
pubmed-meshheading:2178652-Rats, Inbred Strains
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pubmed:year |
1990
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pubmed:articleTitle |
Sandwich enzyme-linked immunosorbent assay of rat apolipoprotein E.
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pubmed:affiliation |
Department of Laboratory Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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