Linked Life Data

2177960

Source:http://linkedlifedata.com/resource/pubmed/id/2177960

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-3-8
pubmed:abstractText
A modification of a protein-binding method (B. L. Brown et al., 1971, Biochem. J. 121, 561, 562) for measurement of adenosine 3',5'-cyclic monophosphate (cAMP) in cell-culture supernatants and urine is described. With filtration over glass-fiber filters and a semiautomatic cell harvester instead of charcoal precipitation as in the original method, free [3H]cAMP tracer was rapidly and uniformly separated from that which was protein-bound. Sensitivity was increased with a high ionic strength buffer and a tritiated cAMP tracer with high specific activity. There was good agreement between cAMP values obtained with the protein-binding method and commercially available radioimmunoassays that were used as reference methods. cAMP could be determined accurately over the range 0.15-8.0 pmol/sample. More than 500 samples could be assayed in duplicate or triplicate in less than 6 h.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:volume
189
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
231-4
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
A modification of a protein-binding method for rapid quantification of cAMP in cell-culture supernatants and body fluid.
pubmed:affiliation
Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't