Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1991-1-29
|
| pubmed:abstractText |
The paper gives a detailed theoretical description of the recently published principle of separation by a dynamic pulse in zone electrophoresis. The theory is illustrated by using a system in which weak acids are separated by migrating against an H+ pulse. The mathematical description pertains to (i) the evolution and migration of the concentration profile of the H+ pulse, and (ii) the migration of a sample substance (here a weak acid) against and through the pulse. As a result the migration (detection) times of the separated sample components are obtained. Based on the theory, a graphical treatment was developed which allows fast optimization of the separation conditions by observing (on the PC monitor) how the trajectories of the substances (drawn in a time vs. distance coordinate system) change with a change in the parameters of the pulse. Some of the results illustrate the possible advantages of using the dynamic pulse method in analytical practice.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0173-0835
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
11
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
724-31
|
| pubmed:dateRevised |
2000-12-18
|
| pubmed:meshHeading | |
| pubmed:year |
1990
|
| pubmed:articleTitle |
Mathematical description of electrophoretic separation of weak acids by means of an H+ pulse.
|
| pubmed:affiliation |
Institute of Analytical Chemistry, Czechoslovak Academy of Sciences, Brno.
|
| pubmed:publicationType |
Journal Article
|