Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
1990-12-4
pubmed:abstractText
We studied the function of the human small (46-kDa) mannose 6-phosphate receptor (SMPR) in transfected mouse L cells that do not express the larger insulin-like growth factor II/mannose 6-phosphate receptor. Cells overexpressing human SMPR were studied for enzyme binding to cell surface receptors, for binding to intracellular receptors in permeabilized cells, and for receptor-mediated endocytosis of recombinant human beta-glucuronidase. Specific binding to human SMPR in permeabilized cells showed a pH optimum between pH 6.0 and pH 6.5. Binding was significant in the presence of EDTA but was enhanced by added divalent cations. Up to 2.3% of the total functional receptor could be detected on the cell surface by enzyme binding. We present experiments showing that at very high levels of overexpression, and at pH 6.5, human SMPR mediated the endocytosis of beta-glucuronidase. At pH 7.5, the rate of endocytosis was only 14% the rate seen at pH 6.5. Cells overexpressing human SMPR also showed reduced secretion of newly synthesized beta-glucuronidase when compared to cells transfected with vector only, suggesting that overexpressed human SMPR can participate in sorting of newly synthesized beta-glucuronidase and partially correct the sorting defect in mouse L cells that do not express the insulin-like growth factor II/mannose 6-phosphate receptor.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-1966041, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-2159321, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-2441386, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-2541923, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-2542255, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-266721, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-2828384, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-2931431, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-2954652, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-2957598, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-2959299, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-2960521, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-2963003, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-2963004, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-2963812, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-2963825, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-2964083, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-2971570, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-2972705, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-3160044, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-3161725, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-6086314, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-6263889, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-6268636, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-6270668, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-6288715, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-6298775, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-6315741, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-6452459, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-6457829, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172972-6768729
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8036-40
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
The overexpressed human 46-kDa mannose 6-phosphate receptor mediates endocytosis and sorting of beta-glucuronidase.
pubmed:affiliation
Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, MO 63104.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.