Linked Life Data

21699758

Source:http://linkedlifedata.com/resource/pubmed/id/21699758

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-6-24
pubmed:abstractText
Early neonatal adaptation to extrauterine life is i.a. dependent on effective mitochondrial biogenesis during foetal development. Understanding of mitochondrial biogenesis is limited, because only scarce data are available from prenatal studies including RNA analyses in human foetal tissues. Aims of the study were focused on the factors affecting RNA quality in human placental tissue (HPT) including temperature, time period before HPT freezing and the Apgar score. In addition, optimal reference genes for mRNA quantification by real-time PCR in HPT were studied. Samples of HPT were obtained after the birth of 20 term neonates. Seven HPT were used for the time-course study of RNA degradation in two different temperatures (0 °C and 24 °C). Various instruments NanoDrop (NanoDrop Technologies), Experion (Bio-Rad Laboratories), Agilent 2100 Bioanalyzer (Agilent Technologies) were used for analysis of RNA integrity, purity and yield. Identification of suitable reference genes was achieved by analysing six candidate genes (ATP5O, SDHA, TBP, HPRT, PMBS, ATP6) for their expression stability (GeNorm application). The results showed that the HPT samples for RNA analyses must be frozen immediately after birth in -80 °C or stored at 0 °C maximally for 1 hour. The reference genes ATP50 and SDHA were the most stable for mRNA quantification in HPT. Human placenta represents easily obtainable source of foetal tissue for studies concerning mitochondrial biogenesis. We demonstrated that the critical limit for optimal storage and handling of HPT are the temperature and the time period before freezing of the samples.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1214-6994
pubmed:author
pubmed:issnType
Print
pubmed:volume
112
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
93-101
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Placental tissue as model for pilot study focused on RNA analysis from human foetal tissue.
pubmed:affiliation
Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't