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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1990-10-4
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pubmed:abstractText |
In muscle cytosolic and glycogen fractions prepared in the presence of 50 mM NaF phosphorylase phosphatase was a approximately 70 kDa complex instead of the 250 kDa or higher seen in the absence of NaF. A approximately 70 kDa complex was also formed when purified 37 kDa phosphatase-1 catalytic subunit (but not its 33 kDa tryptic fragment) was exposed to NaF. Treating this latter complex with a cross-linker led to disappearance of the 37 kDa protein and formation of a approximately 66 kDa band (detected by SDS electrophoresis), thus indicating the dimeric nature of the approximately 70 kDa complex.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
171
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
362-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:2168178-Animals,
pubmed-meshheading:2168178-Chromatography, Gel,
pubmed-meshheading:2168178-Macromolecular Substances,
pubmed-meshheading:2168178-Molecular Weight,
pubmed-meshheading:2168178-Muscles,
pubmed-meshheading:2168178-Phosphoprotein Phosphatases,
pubmed-meshheading:2168178-Phosphorylase Phosphatase,
pubmed-meshheading:2168178-Protein Phosphatase 1,
pubmed-meshheading:2168178-Rabbits,
pubmed-meshheading:2168178-Sodium Fluoride,
pubmed-meshheading:2168178-Structure-Activity Relationship,
pubmed-meshheading:2168178-Trypsin
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pubmed:year |
1990
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pubmed:articleTitle |
Effect of NaF on type-1 phosphatase aggregation.
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pubmed:affiliation |
Ist. di Patologia Generale, Universita di Pisa, Italy.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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