Linked Life Data

2166940

Source:http://linkedlifedata.com/resource/pubmed/id/2166940

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
1990-9-20
pubmed:abstractText
In the preceding paper we described an experiment that determined the in vivo forward mutation rate in a single replication cycle for spleen necrosis virus. In addition to substitutions, frameshifts, and hypermutations, the mutated proviruses contained two classes of deletions. One class of deletions contained short direct repeats at the deletion junctions. Another class of deletions had short stretches of sequences inserted at the deletion junctions. In this report, we describe the deletion mutations, and we present models for their generation. Detailed analysis of two deletions with insertions indicates that these mutations occurred as a result of template switching during plus-strand DNA synthesis. The analysis also indicates that fragments of viral RNA generated by the viral RNase H endonuclease are used as templates and contribute to the sequences inserted at the deletion junctions.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2166940-2201018, http://linkedlifedata.com/resource/pubmed/commentcorrection/2166940-2501754, http://linkedlifedata.com/resource/pubmed/commentcorrection/2166940-2985470, http://linkedlifedata.com/resource/pubmed/commentcorrection/2166940-2989561, http://linkedlifedata.com/resource/pubmed/commentcorrection/2166940-3122035, http://linkedlifedata.com/resource/pubmed/commentcorrection/2166940-3287617, http://linkedlifedata.com/resource/pubmed/commentcorrection/2166940-3368459, http://linkedlifedata.com/resource/pubmed/commentcorrection/2166940-3422416, http://linkedlifedata.com/resource/pubmed/commentcorrection/2166940-3796606, http://linkedlifedata.com/resource/pubmed/commentcorrection/2166940-5439728, http://linkedlifedata.com/resource/pubmed/commentcorrection/2166940-6283110, http://linkedlifedata.com/resource/pubmed/commentcorrection/2166940-6302692
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6024-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Broad spectrum of in vivo forward mutations, hypermutations, and mutational hotspots in a retroviral shuttle vector after a single replication cycle: deletions and deletions with insertions.
pubmed:affiliation
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't