Linked Life Data

21641386

Source:http://linkedlifedata.com/resource/pubmed/id/21641386

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2011-6-6
pubmed:abstractText
Amyloid deposition and reduced ?-cell mass are pathological hallmarks of the pancreatic islet in type 2 diabetes; however, whether the extent of amyloid deposition is associated with decreased ?-cell mass is debated. We investigated the possible relationship and, for the first time, determined whether increased islet amyloid and/or decreased ?-cell area quantified on histological sections is correlated with increased ?-cell apoptosis. Formalin-fixed, paraffin-embedded human pancreas sections from subjects with (n = 29) and without (n = 39) diabetes were obtained at autopsy (64 ± 2 and 70 ± 4 islets/subject, respectively). Amyloid and ? cells were visualized by thioflavin S and insulin immunolabeling. Apoptotic ? cells were detected by colabeling for insulin and by TUNEL. Diabetes was associated with increased amyloid deposition, decreased ?-cell area, and increased ?-cell apoptosis, as expected. There was a strong inverse correlation between ?-cell area and amyloid deposition (r = -0.42, P < 0.001). ?-Cell area was selectively reduced in individual amyloid-containing islets from diabetic subjects, compared with control subjects, but amyloid-free islets had ?-cell area equivalent to islets from control subjects. Increased amyloid deposition was associated with ?-cell apoptosis (r = 0.56, P < 0.01). Thus, islet amyloid is associated with decreased ?-cell area and increased ?-cell apoptosis, suggesting that islet amyloid deposition contributes to the decreased ?-cell mass that characterizes type 2 diabetes.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1525-2191
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
178
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2632-40
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
?-cell loss and ?-cell apoptosis in human type 2 diabetes are related to islet amyloid deposition.
pubmed:affiliation
Division of Metabolism, Endocrinology and Nutrition, Veterans Affairs Puget Sound Health Care System and University of Washington, Seattle, Washington, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural