Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2011-3-14
pubmed:abstractText
Immune responses against the Leishmania antigens are not sufficient to protect against a leishmania challenge. Therefore these antigens need to be potentiated by various adjuvants and delivery systems. In this study, Poly (d,l-lactide-co-glycolide (PLGA) nanospheres as antigen delivery system and Quillaja saponins (QS) as immunoadjuvant have been used to enhance the immune response against autoclaved Leishmania major (ALM). PLGA nanospheres were prepared by a double-emulsion (W/O/W) technique. Particulate characteristics were studied by scanning electron microscopy and particle size analysis. Mean diameter for nanospheres loaded with ALM+QS was 294 ± 106 nm. BALB/c mice were immunized three times in 3-weeks intervals using ALM plus QS loaded nanospheres [(ALM+QS)PLGA], ALM encapsulated with PLGA nanospheres [(ALM)PLGA], (ALM)PLGA + QS, ALM + QS, ALM alone or PBS. The intensity of infection induced by L. major challenge was assessed by measuring size of footpad swelling. The strongest protection, showed by significantly (P < 0.05) smaller footpad, were observed in mice immunized with (ALM)PLGA. The (ALM+QS)PLGA group showed the least protection and highest swelling, while the (ALM)PLGA+QS, ALM+QS and ALM showed an intermediate protection with no significant difference. The mice immunized with ALM and ALM+QS showed the highest IgG2a/IgG1 ratio (P < 0.01), followed by (ALM)PLGA+QS. The highest IFN-? and lowest IL-4 production was seen in (ALM)PLGA+QS, ALM+QS groups. The highest parasite burden was observed in (ALM)PLGA+QS and (ALM+QS)PLGA groups. It is concluded that PLGA nanospheres as a vaccine delivery system could increase the protective immune responses, but QS adjuvant has a reverse effect on protective immune responses and the least protective responses were seen in the presence of this adjuvant.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic, http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Protozoan, http://linkedlifedata.com/resource/pubmed/chemical/Drug Carriers, http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G, http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4, http://linkedlifedata.com/resource/pubmed/chemical/Lactic Acid, http://linkedlifedata.com/resource/pubmed/chemical/Leishmaniasis Vaccines, http://linkedlifedata.com/resource/pubmed/chemical/Polyglycolic Acid, http://linkedlifedata.com/resource/pubmed/chemical/Saponins, http://linkedlifedata.com/resource/pubmed/chemical/autoclaved Leishmania major vaccine, http://linkedlifedata.com/resource/pubmed/chemical/polylactic acid-polyglycolic acid...
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0127-5720
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
639-50
pubmed:meshHeading
pubmed-meshheading:21399606-Adjuvants, Immunologic, pubmed-meshheading:21399606-Animals, pubmed-meshheading:21399606-Antibodies, Protozoan, pubmed-meshheading:21399606-Drug Carriers, pubmed-meshheading:21399606-Drug Delivery Systems, pubmed-meshheading:21399606-Female, pubmed-meshheading:21399606-Foot, pubmed-meshheading:21399606-Immunization, pubmed-meshheading:21399606-Immunoglobulin G, pubmed-meshheading:21399606-Interferon-gamma, pubmed-meshheading:21399606-Interleukin-4, pubmed-meshheading:21399606-Lactic Acid, pubmed-meshheading:21399606-Leishmaniasis, pubmed-meshheading:21399606-Leishmaniasis Vaccines, pubmed-meshheading:21399606-Leukocytes, Mononuclear, pubmed-meshheading:21399606-Mice, pubmed-meshheading:21399606-Mice, Inbred BALB C, pubmed-meshheading:21399606-Microscopy, Electron, Scanning, pubmed-meshheading:21399606-Nanospheres, pubmed-meshheading:21399606-Polyglycolic Acid, pubmed-meshheading:21399606-Quillaja, pubmed-meshheading:21399606-Saponins
pubmed:year
2010
pubmed:articleTitle
Immunization against leishmaniasis by PLGA nanospheres loaded with an experimental autoclaved Leishmania major (ALM) and Quillaja saponins.
pubmed:affiliation
Nanotechnology Research Center and School of Pharmacy, Mashhad University of Medical Sciences, PO Box 91775-1365, Mashhad, Iran.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't