Linked Life Data

21356843

Source:http://linkedlifedata.com/resource/pubmed/id/21356843

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2011-3-1
pubmed:abstractText
INTRODUCTIONThis protocol describes a method for performing reduction and S-alkylation of Coomassie-blue-stained proteins within an intact gel. Reduction is performed with dithiothreitol, and alkylation with 4-vinylpyridine. (Treatment of free cysteines with 4-vinylpyridine yields the S-?-(4-pyridylethyl) cysteinyl derivative.) S-?-(4-pyridylethyl) cysteine-containing peptides can be readily identified during RP-HPLC by their characteristic absorbance at 254 nm and during electrospray ionization tandem mass spectrometry by the appearance of a characteristic pyridylethyl fragment ion of 10(6) Da. The position of cysteine residues in a polypeptide sequence can be determined either by mass spectrometry or as phenylthiohydantoin S-?-(4-pyridylethyl) cysteine during Edman degradation.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:author
pubmed:volume
2008
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
pdb.prot4604
pubmed:year
2008
pubmed:articleTitle
In-gel s-pyridylethylation of gel-resolved proteins: whole gel method.
pubmed:publicationType
Journal Article