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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2011-3-7
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pubmed:abstractText |
Transcriptional repression is essential for establishing precise patterns of gene expression during development. Repressors governing early Drosophila segmentation can be classified as short- or long-range factors based on their ranges of action, acting either locally to quench adjacent activators or broadly to silence an entire locus. Paradoxically, these repressors recruit common corepressors, Groucho and CtBP, despite their different ranges of repression. To reveal the mechanisms underlying these two distinct modes of repression, we performed chromatin analysis using the prototypical long-range repressor Hairy and the short-range repressor Knirps. Chromatin immunoprecipitation and micrococcal nuclease mapping studies reveal that Knirps causes local changes of histone density and acetylation, and the inhibition of activator recruitment, without affecting the recruitment of basal transcriptional machinery. In contrast, Hairy induces widespread histone deacetylation and inhibits the recruitment of basal machinery without inducing chromatin compaction. Our study provides detailed mechanistic insight into short- and long-range repression on selected endogenous target genes and suggests that the transcriptional corepressors can be differentially deployed to mediate chromatin changes in a context-dependent manner.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alcohol Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/Basic Helix-Loop-Helix...,
http://linkedlifedata.com/resource/pubmed/chemical/C-terminal binding protein,
http://linkedlifedata.com/resource/pubmed/chemical/Chromatin,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Histones,
http://linkedlifedata.com/resource/pubmed/chemical/Micrococcal Nuclease,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/groucho protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/hairy protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/kni protein, Drosophila
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1879-0445
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2011 Elsevier Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
8
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
406-12
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:21353562-Acetylation,
pubmed-meshheading:21353562-Alcohol Oxidoreductases,
pubmed-meshheading:21353562-Animals,
pubmed-meshheading:21353562-Basic Helix-Loop-Helix Transcription Factors,
pubmed-meshheading:21353562-Chromatin,
pubmed-meshheading:21353562-Chromatin Immunoprecipitation,
pubmed-meshheading:21353562-DNA-Binding Proteins,
pubmed-meshheading:21353562-Drosophila,
pubmed-meshheading:21353562-Drosophila Proteins,
pubmed-meshheading:21353562-Gene Expression Regulation, Developmental,
pubmed-meshheading:21353562-Histones,
pubmed-meshheading:21353562-In Situ Hybridization,
pubmed-meshheading:21353562-Micrococcal Nuclease,
pubmed-meshheading:21353562-Plasmids,
pubmed-meshheading:21353562-Polymerase Chain Reaction,
pubmed-meshheading:21353562-Repressor Proteins,
pubmed-meshheading:21353562-Silencer Elements, Transcriptional
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pubmed:year |
2011
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pubmed:articleTitle |
Long- and short-range transcriptional repressors induce distinct chromatin states on repressed genes.
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pubmed:affiliation |
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824-1319, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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