| pubmed-article:21300643 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21300643 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:21300643 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:21300643 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:21300643 | lifeskim:mentions | umls-concept:C0017428 | lld:lifeskim |
| pubmed-article:21300643 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:21300643 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:21300643 | lifeskim:mentions | umls-concept:C0049241 | lld:lifeskim |
| pubmed-article:21300643 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:21300643 | pubmed:dateCreated | 2011-4-26 | lld:pubmed |
| pubmed-article:21300643 | pubmed:abstractText | Recently, 5-hydroxymethylcytosine (5hmC) was identified in mammalian genomic DNA. The biological role of this modification remains unclear; however, identifying the genomic location of this modified base will assist in elucidating its function. We describe a method for the rapid and inexpensive identification of genomic regions containing 5hmC. This method involves the selective glucosylation of 5hmC residues by the ?-glucosyltransferase from T4 bacteriophage creating ?-glucosyl-5-hydroxymethylcytosine (?-glu-5hmC). The ?-glu-5hmC modification provides a target that can be efficiently and selectively pulled down by J-binding protein 1 coupled to magnetic beads. DNA that is precipitated is suitable for analysis by quantitative PCR, microarray or sequencing. Furthermore, we demonstrate that the J-binding protein 1 pull down assay identifies 5hmC at the promoters of developmentally regulated genes in human embryonic stem cells. The method described here will allow for a greater understanding of the temporal and spatial effects that 5hmC may have on epigenetic regulation at the single gene level. | lld:pubmed |
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| pubmed-article:21300643 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21300643 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21300643 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:21300643 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21300643 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:21300643 | pubmed:issn | 1362-4962 | lld:pubmed |
| pubmed-article:21300643 | pubmed:author | pubmed-author:KrokanHans... | lld:pubmed |
| pubmed-article:21300643 | pubmed:author | pubmed-author:KlunglandArne... | lld:pubmed |
| pubmed-article:21300643 | pubmed:author | pubmed-author:VågbøCathrine... | lld:pubmed |
| pubmed-article:21300643 | pubmed:author | pubmed-author:TripathiPanka... | lld:pubmed |
| pubmed-article:21300643 | pubmed:author | pubmed-author:RobertsonAdam... | lld:pubmed |
| pubmed-article:21300643 | pubmed:author | pubmed-author:DahlJohn AJA | lld:pubmed |
| pubmed-article:21300643 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21300643 | pubmed:volume | 39 | lld:pubmed |
| pubmed-article:21300643 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21300643 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21300643 | pubmed:pagination | e55 | lld:pubmed |
| pubmed-article:21300643 | pubmed:dateRevised | 2011-7-28 | lld:pubmed |
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| pubmed-article:21300643 | pubmed:meshHeading | pubmed-meshheading:21300643... | lld:pubmed |
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| pubmed-article:21300643 | pubmed:meshHeading | pubmed-meshheading:21300643... | lld:pubmed |
| pubmed-article:21300643 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21300643 | pubmed:articleTitle | A novel method for the efficient and selective identification of 5-hydroxymethylcytosine in genomic DNA. | lld:pubmed |
| pubmed-article:21300643 | pubmed:affiliation | Centre for Molecular Biology and Neuroscience and Institute of Medical Microbiology, Oslo University Hospital, Rikshospitalet, Norway. | lld:pubmed |
| pubmed-article:21300643 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21300643 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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