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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2011-4-26
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pubmed:abstractText |
Recently, 5-hydroxymethylcytosine (5hmC) was identified in mammalian genomic DNA. The biological role of this modification remains unclear; however, identifying the genomic location of this modified base will assist in elucidating its function. We describe a method for the rapid and inexpensive identification of genomic regions containing 5hmC. This method involves the selective glucosylation of 5hmC residues by the ?-glucosyltransferase from T4 bacteriophage creating ?-glucosyl-5-hydroxymethylcytosine (?-glu-5hmC). The ?-glu-5hmC modification provides a target that can be efficiently and selectively pulled down by J-binding protein 1 coupled to magnetic beads. DNA that is precipitated is suitable for analysis by quantitative PCR, microarray or sequencing. Furthermore, we demonstrate that the J-binding protein 1 pull down assay identifies 5hmC at the promoters of developmentally regulated genes in human embryonic stem cells. The method described here will allow for a greater understanding of the temporal and spatial effects that 5hmC may have on epigenetic regulation at the single gene level.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-10562569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-11700315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-13753193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-1706062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-18729733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-18771397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-19208222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-19372391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-19372393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-19376112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-20371518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-20453866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-20569209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-20583021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-20639862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-20685817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-21057493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-2999696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-4935677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-8261512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21300643-9774669
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
e55
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pubmed:dateRevised |
2011-7-28
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pubmed:meshHeading |
pubmed-meshheading:21300643-Carrier Proteins,
pubmed-meshheading:21300643-Cytosine,
pubmed-meshheading:21300643-DNA,
pubmed-meshheading:21300643-Embryonic Stem Cells,
pubmed-meshheading:21300643-Gene Expression Regulation,
pubmed-meshheading:21300643-Genome, Human,
pubmed-meshheading:21300643-Genomics,
pubmed-meshheading:21300643-Glucosyltransferases,
pubmed-meshheading:21300643-Humans,
pubmed-meshheading:21300643-Promoter Regions, Genetic,
pubmed-meshheading:21300643-Protozoan Proteins
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pubmed:year |
2011
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pubmed:articleTitle |
A novel method for the efficient and selective identification of 5-hydroxymethylcytosine in genomic DNA.
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pubmed:affiliation |
Centre for Molecular Biology and Neuroscience and Institute of Medical Microbiology, Oslo University Hospital, Rikshospitalet, Norway.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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