Linked Life Data

21154530

Source:http://linkedlifedata.com/resource/pubmed/id/21154530

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-12-14
pubmed:abstractText
The efficiency of chemical RNA synthesis has been radically improved by the use of pivaloyloxymethyl (PivOM) groups as 2'-protection, containing an acetal spacer that minimizes the steric effect of the ester group on the neighboring amidite during the coupling. However, the major benefit of the base-labile PivOM groups is their simple removal upon standard basic conditions applied to deprotect the RNA and release it from solid supports. Combined with standard acyl groups for nucleobases, cyanoethyl groups for phosphates, and base-cleavable linkers, PivOM groups make RNA deprotection as simple as DNA deprotection. Thus, no additional deprotection step with tedious desalting workup procedures is required, and RNA synthesis becomes as convenient and efficient as DNA synthesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1934-9289
pubmed:author
pubmed:copyrightInfo
© 2010 by John Wiley & Sons, Inc.
pubmed:issnType
Electronic
pubmed:volume
Chapter 3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit3.19
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Chemical synthesis of RNA with base-labile 2'-o-(pivaloyloxymethyl)-protected ribonucleoside phosphoramidites.
pubmed:affiliation
IBMM, University of Montpellier, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't