Linked Life Data

21126573

Source:http://linkedlifedata.com/resource/pubmed/id/21126573

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-3-7
pubmed:abstractText
Resveratrol is a phytoalexin with antioxidant properties. We evaluated resveratrol toxicity in swim-up selected human sperm and in rat spermatocytes and spermatids separated by the STAPUT method. Resveratrol antioxidant activity was tested against lipid peroxidation (LPO) induced by tert-butyl hydroperoxide. LPO was evaluated using the C11-BODIPY(581/591) probe and transmission electron microscopy in samples incubated with and without resveratrol. LD50 for human sperm and rat spermatids was 50 ?M; spermatocytes were more sensitive to resveratrol cytotoxicity. Sperm motility increased progressively at 30 ?M, 15 ?M and 6 ?M. 15 ?M resveratrol acts against LPO, preserving sperm chromatin and plasma membranes. LPO were more marked in spermatocytes than in spermatids and the effect of resveratrol was more evident in spermatocytes. In this study, the scavenger properties of resveratrol were demonstrated in vitro in human sperm and rat germ cells, thus resveratrol could be added to the media used in assisted reproduction techniques and cryopreservation when oxidative stress is exacerbated.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1873-1708
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
239-46
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Effect of trans-resveratrol on induced oxidative stress in human sperm and in rat germinal cells.
pubmed:affiliation
Department Biomedical Sciences, Applied Biology Section, University of Siena, Policlinico Santa Maria alle Scotte, Viale Bracci, 53100 Siena, Italy.
pubmed:publicationType
Journal Article