Linked Life Data

21107719

Source:http://linkedlifedata.com/resource/pubmed/id/21107719

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2011-6-15
pubmed:abstractText
In fission yeast Schizosaccharomyces pombe, the directions of cell growth change from a monopolar manner to a bipolar manner, which is known as 'New End Take Off' (NETO). We previously found that Arf6, a member (class III) of the ADP-ribosylation factor GTPase (Arf) family, is necessary for NETO in fission yeast. Here we report the characterization of a S. pombe gene, ucp3, encoding a putative Arf GTPase-activating protein (GAP) for Arf6. The Ucp3 contains Arf GAP domain, and has a high similarity to Gts1, which was identified as a GAP for Arf3 (class III Arf) in Saccharomyces cerevisiae. Overexpression of ucp3 inhibited growth from new end possibly by disturbing the GDP/GTP-cycling of Arf6. Gene disruption of ucp3 revealed that Ucp3 is essential for cell viability. Ucp3 uniformly localizes to the cell periphery. And its localization is not dependent on microtubules, actin cytoskeletons, Arf6 and Syt22 (guanine nucleotide exchange factor for Arf6). We hypothesize that Ucp3 functions as a GAP for Arf6. Moreover, Ucp3 might have another function important for cell viability.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1573-4978
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3875-82
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Fission yeast ucp3 gene encodes a putative Arf6 GTPase-activating protein.
pubmed:affiliation
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Higashi 1-1-1, Tsukuba, 305-8566, Japan. a-fujita@aist.go.jp
pubmed:publicationType
Journal Article