Source:http://linkedlifedata.com/resource/pubmed/id/21056546
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2010-12-14
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| pubmed:abstractText |
The hypocretin (also known as orexin) neuropeptide system coordinates the regulation of various physiological processes. A reduction in Nr6a1 expression was observed in hypocretin neuron-ablated transgenic mice. To show that prepro-hypocretin transcription is functionally modulated by NR6A1, we performed chromatin immunoprecipitation (ChIP) analysis, double-immunostaining, a luciferase reporter assay, and an in utero electroporation study. ChIP analysis showed that endogenous NR6A1 binds to a putative NR6A1-binding site. Double-immunostaining indicated almost all hypocretin neurons were positive for NR6A1 immunoreactivity. NR6A1 overexpression in SH-SY5Y cells modulated hypocretin promoter activity, an effect that was countered by lacking a putative NR6A1-binding site. Electroporation with Nr6a1 in the foetal hypothalamus promoted hypocretin transcription as compared to GFP-electroporation. These experiments confirmed that NR6A1 works as a regulator for hypocretin transcription.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
1090-2104
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright © 2010 Elsevier Inc. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:day |
10
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| pubmed:volume |
403
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
178-83
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| pubmed:dateRevised |
2011-10-6
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| pubmed:meshHeading |
pubmed-meshheading:21056546-Animals,
pubmed-meshheading:21056546-Base Sequence,
pubmed-meshheading:21056546-Cell Line,
pubmed-meshheading:21056546-DNA Mutational Analysis,
pubmed-meshheading:21056546-Gene Expression Regulation,
pubmed-meshheading:21056546-Humans,
pubmed-meshheading:21056546-Hypothalamus,
pubmed-meshheading:21056546-Immunoprecipitation,
pubmed-meshheading:21056546-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:21056546-Male,
pubmed-meshheading:21056546-Mice,
pubmed-meshheading:21056546-Mice, Inbred C57BL,
pubmed-meshheading:21056546-Molecular Sequence Data,
pubmed-meshheading:21056546-Neuropeptides,
pubmed-meshheading:21056546-Nuclear Receptor Subfamily 6, Group A, Member 1,
pubmed-meshheading:21056546-Promoter Regions, Genetic,
pubmed-meshheading:21056546-Sequence Deletion,
pubmed-meshheading:21056546-Transcription, Genetic
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| pubmed:year |
2010
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| pubmed:articleTitle |
Transcriptional regulation of the hypocretin/orexin gene by NR6A1.
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| pubmed:affiliation |
Department of Sleep Disorders Research, Tokyo Institute of Psychiatry, Tokyo, Japan. tanaka-ss@igakuken.or.jp
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|