Linked Life Data

21050046

Source:http://linkedlifedata.com/resource/pubmed/id/21050046

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2010-11-5
pubmed:abstractText
Mud crab dicistrovirus (MCDV) is a recently identified single-stranded positive RNA virus, which causes serious economic loss. The three structural proteins of MCDV were separated by SDS-PAGE. In this study, a recombinant MCDV-VP3 was successfully expressed by an Escherichia coli expression system. After immunization and cell fusion, three mouse hybridomas (1G6H9, 1E7B8, 4B6E10) producing MAbs against MCDV-VP3 were established. The MAbs obtained were fully characterized using indirect ELISA and Western blot analysis. The ELISA results showed that the titers of MAbs were in the range of 1:3.200×10(3) in culture fluids and 1:2.048×10(5) in ascitic fluids. Using Western blot analyses, we observed the specific characteristic band that defines VP3. It demonstrated that all the MAbs were directed against MCDV-VP3. The results of immunogold transmission electron microscopy (IEM) suggested that MCDV-VP3 is the capsid protein of MCDV. The preparation of MAbs specific to the structure protein of MCDV would be useful for studying the function of the structure proteins and the mechanism of infection and pathogenesis of MCDV.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1557-8348
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
437-40
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Monoclonal antibodies produced against VP3 of a novel mud crab dicistrovirus.
pubmed:affiliation
State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen Zhongshan University, Guangzhou, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies