Source:http://linkedlifedata.com/resource/pubmed/id/21046333
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| rdf:type | |
| lifeskim:mentions |
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umls-concept:C2936389
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| pubmed:issue |
5
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| pubmed:dateCreated |
2010-11-3
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| pubmed:abstractText |
Microbial communities in hot pepper (Capsicum annuum L.) cultivation fields under different cultivation methods were investigated by terminal restriction fragment length polymorphism (T-RFLP) analysis. Rhizosphere soil and leaf samples were collected from control, conventional and nature-friendly cultivation fields between May and July, 2009. Two Bacillus subtilis strains were applied to nature-friendly cultivation fields as biocontrol agents during the sampling period. Relative abundances of bacteria and plant pathogenic fungi related T-RFs were also measured to monitor the effect of biocontrol agents on potential plant pathogenic fungi. In the principal component analysis (PCA) based on T-RFLP profiles, the microbial communities from rhizosphere soil samples in July, including bacteria and fungi, showed distinct difference between nature-friendly cultivation fields and other cultivation fields. However, there was no correlation between cultivation methods and leaf microbial communities at any sampling period. Changes in the abundance of bacteria related T-RF in the rhizosphere of nature-friendly cultivation fields were observed clearly two months after application of biocontrol agent, while the abundance of plant pathogenic fungi related T-RFs significantly decreased.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
1976-3794
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
48
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
566-72
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| pubmed:meshHeading |
pubmed-meshheading:21046333-Biodiversity,
pubmed-meshheading:21046333-Capsicum,
pubmed-meshheading:21046333-DNA Fingerprinting,
pubmed-meshheading:21046333-Metagenome,
pubmed-meshheading:21046333-Pest Control, Biological,
pubmed-meshheading:21046333-Plant Leaves,
pubmed-meshheading:21046333-Plant Roots,
pubmed-meshheading:21046333-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:21046333-Rhizosphere,
pubmed-meshheading:21046333-Soil Microbiology
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| pubmed:year |
2010
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| pubmed:articleTitle |
Application of terminal restriction fragment length polymorphism (T-RFLP) analysis to monitor effect of biocontrol agents on rhizosphere microbial community of hot pepper (Capsicum annuum L.).
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| pubmed:affiliation |
Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|