Linked Life Data

20951242

Source:http://linkedlifedata.com/resource/pubmed/id/20951242

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2011-2-14
pubmed:abstractText
Protein kinase C (PKC) plays an essential role in cellular signal transduction for mediating a variety of biological functions. There are 11 PKC isoforms and these isoforms are believed to play distinct roles in cells. Although the role of individual isoforms of PKC has been investigated in many fields, little is known about the role of PKC in osteoblastic differentiation. Here, we investigated which isoforms of PKC are involved in osteoblastic differentiation of the mouse preosteoblastic cell line MC3T3-E1. Treatment with Gö6976, an inhibitor of PKC? and PKC?I, increased alkaline phosphatase (ALP) activity as well as gene expression of ALP and Osteocalcin (OCN), and enhanced calcification of the extracellular matrix. Concurrently, osteoblastic cell proliferation decreased at a concentration of 1.0 ?M. In contrast, a PKC? inhibitor, which inhibits PKC?I and PKC?II, did not significantly affect osteoblastic differentiation or cell proliferation. Knockdown of PKC? using MC3T3-E1 cells transfected with siRNA also induced an increase in ALP activity and in gene expression of ALP and OCN. In contrast, overexpression of wild-type PKC? decreased ALP activity and attenuated osteoblastic differentiation markers including ALP and OCN, but promoted cell proliferation. Taken together, our results indicate that PKC? suppresses osteoblastic differentiation, but promotes osteoblastic cell proliferation. These results imply that PKC? may have a pivotal role in cell signaling that modulates the differentiation and proliferation of osteoblasts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1873-2763
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
476-84
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
PKC? suppresses osteoblastic differentiation.
pubmed:affiliation
Department of Orthopaedic Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan. nakura-osaka@umin.ac.jp
pubmed:publicationType
Journal Article