Linked Life Data

20950277

Source:http://linkedlifedata.com/resource/pubmed/id/20950277

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-12-16
pubmed:abstractText
PPAR (peroxisome-proliferator-activated receptor) ?, a nuclear receptor, can be conjugated with SUMO (small ubiquitin-like modifier), which results in the negative regulation of its transcriptional activity. In the present study, we tested whether de-SUMOylation of PPAR? affects the expression of PPAR? target genes in mouse muscle cells and investigated the mechanism by which de-SUMOylation increases PPAR? transcriptional activity. We found that the SUMO-specific protease SENP2 [SUMO1/sentrin/SMT3 (suppressor of mif two 3 homologue 1)-specific peptidase 2] effectively de-SUMOylates PPAR?-SUMO conjugates. Overexpression of SENP2 in C2C12 cells increased the expression of some PPAR? target genes, such as FABP3 (fatty-acid-binding protein 3) and CD36 (fatty acid translocase), both in the absence and presence of rosiglitazone. In contrast, overexpression of SENP2 did not affect the expression of another PPAR? target gene ADRP (adipose differentiation-related protein). De-SUMOylation of PPAR? increased ChIP (chromatin immunoprecipitation) of both a recombinant PPRE (PPAR-response element) and endogenous PPREs of the target genes CD36 and FABP3, but ChIP of the PPRE in the ADRP promoter was not affected by SENP2 overexpression. In conclusion, these results indicate that SENP2 de-SUMOylates PPAR? in myotubes, and de-SUMOylation of PPAR? selectively increases the expression of some PPAR? target genes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1470-8728
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
433
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
155-61
pubmed:dateRevised
2011-9-6
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
SUMO modification selectively regulates transcriptional activity of peroxisome-proliferator-activated receptor ? in C2C12 myotubes.
pubmed:affiliation
Department of Internal Medicine, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-744, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't