| pubmed-article:20949323 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20949323 | lifeskim:mentions | umls-concept:C1333166 | lld:lifeskim |
| pubmed-article:20949323 | lifeskim:mentions | umls-concept:C1705705 | lld:lifeskim |
| pubmed-article:20949323 | lifeskim:mentions | umls-concept:C0334227 | lld:lifeskim |
| pubmed-article:20949323 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:20949323 | lifeskim:mentions | umls-concept:C1332813 | lld:lifeskim |
| pubmed-article:20949323 | lifeskim:mentions | umls-concept:C0073096 | lld:lifeskim |
| pubmed-article:20949323 | lifeskim:mentions | umls-concept:C2349975 | lld:lifeskim |
| pubmed-article:20949323 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:20949323 | pubmed:dateCreated | 2011-3-29 | lld:pubmed |
| pubmed-article:20949323 | pubmed:abstractText | Vasopressin-activated calcium-mobilizing (VACM-1) protein is a cul-5 gene product that forms complexes with a subclass of ubiquitin E3 ligases involved in proteasomal protein degradation. The expression of VACM-1 cDNA in the T47D breast cancer cell line inhibits growth and decreases phosphorylation of mitogen activated protein kinase. Factors that regulate expression or stability of VACM-1 protein have not been identified, however. In our search to identify drugs/substances that may control VACM-1 protein expression, we examined the effects of resveratrol (trans-3,5,4'-trihydroxystilbene), a natural component in the human diet which inhibits tumor initiation and promotion. CMV vector and VACM-1 cDNA stably transfected T47D breast cancer-derived cells were treated with resveratrol and cell growth and VACM-1 protein concentrations were measured. Since the cellular mechanism of resveratrol-dependent inhibition of cell growth also involves the regulation of estrogen receptors, the effect of 17-?-estradiol and resveratrol on ER? levels and on cell growth was examined in control and in VACM-1 cDNA transfected cells. Our results demonstrate that antiproliferative effect of resveratrol observed in the control T47D cancer cells was significantly enhanced in VACM-1 cDNA transfected T47D cells. Western blot results indicated that resveratrol increased VACM-1 protein concentration. Finally, treatment with resveratrol for 24 and 48 h attenuated 17-?-estradiol induced increase in cell growth both in control and in VACM-1 cDNA transfected cells. The effect was significantly higher in the VACM-1 cDNA transfected cells when compared to controls. These results indicate that the antiproliferative effect of resveratrol may involve induction of VACM-1/cul5. | lld:pubmed |
| pubmed-article:20949323 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20949323 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20949323 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20949323 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20949323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20949323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20949323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20949323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20949323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20949323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20949323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20949323 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20949323 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:20949323 | pubmed:issn | 1573-6822 | lld:pubmed |
| pubmed-article:20949323 | pubmed:author | pubmed-author:LewisStevenS | lld:pubmed |
| pubmed-article:20949323 | pubmed:author | pubmed-author:Burnatowska-H... | lld:pubmed |
| pubmed-article:20949323 | pubmed:author | pubmed-author:JohnsonAlyssa... | lld:pubmed |
| pubmed-article:20949323 | pubmed:author | pubmed-author:HledinMichael... | lld:pubmed |
| pubmed-article:20949323 | pubmed:author | pubmed-author:MarquezGabrie... | lld:pubmed |
| pubmed-article:20949323 | pubmed:author | pubmed-author:HarperEmilyE | lld:pubmed |
| pubmed-article:20949323 | pubmed:author | pubmed-author:LubbersJustin... | lld:pubmed |
| pubmed-article:20949323 | pubmed:author | pubmed-author:GravesDanelle... | lld:pubmed |
| pubmed-article:20949323 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20949323 | pubmed:volume | 27 | lld:pubmed |
| pubmed-article:20949323 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20949323 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20949323 | pubmed:pagination | 95-105 | lld:pubmed |
| pubmed-article:20949323 | pubmed:meshHeading | pubmed-meshheading:20949323... | lld:pubmed |
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| pubmed-article:20949323 | pubmed:meshHeading | pubmed-meshheading:20949323... | lld:pubmed |
| pubmed-article:20949323 | pubmed:meshHeading | pubmed-meshheading:20949323... | lld:pubmed |
| pubmed-article:20949323 | pubmed:meshHeading | pubmed-meshheading:20949323... | lld:pubmed |
| pubmed-article:20949323 | pubmed:meshHeading | pubmed-meshheading:20949323... | lld:pubmed |
| pubmed-article:20949323 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:20949323 | pubmed:articleTitle | Resveratrol enhances anti-proliferative effect of VACM-1/cul5 in T47D cancer cells. | lld:pubmed |
| pubmed-article:20949323 | pubmed:affiliation | Department of Biology, Hope College, Holland, MI 49422-9000, USA. | lld:pubmed |
| pubmed-article:20949323 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20949323 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
