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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0017262,
umls-concept:C0031727,
umls-concept:C0034817,
umls-concept:C0109317,
umls-concept:C0127400,
umls-concept:C0333348,
umls-concept:C0441655,
umls-concept:C0521119,
umls-concept:C0752312,
umls-concept:C0851285,
umls-concept:C0887899,
umls-concept:C1150579,
umls-concept:C1155003,
umls-concept:C1171362,
umls-concept:C1333340,
umls-concept:C1366882,
umls-concept:C1370600,
umls-concept:C1420816,
umls-concept:C1515670,
umls-concept:C1521761,
umls-concept:C1705767,
umls-concept:C1705791,
umls-concept:C1709059
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pubmed:issue |
3
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pubmed:dateCreated |
2010-11-5
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pubmed:abstractText |
The immune receptor expressed on myeloid cells 1 (IREM-1) has been known to regulate the activities of myeloid cells through its immunoreceptor tyrosine-based inhibition motifs (ITIMs) in its intracellular region. In order to investigate its effect on macrophage activation, a human macrophage cell line (THP-1) was tested after stimulation of its membrane-bound form of B cell activation factor (BAFF), which has been shown to modulate inflammatory activities through induction of proinflammatory mediator expression and suppression of phagocytosis. IREM-1-specific monoclonal antibodies detected the expression of high levels of IREM-1 in THP-1 cells. Cross-linking of IREM-1 with these antibodies resulted in the blockage of the BAFF-mediated expression of interleukin (IL)-8 and matrix metalloproteinase (MMP)-9 through inhibition of the activation of extracellular regulated kinase (ERK) and phosphorylation/degradation of I?B. Furthermore, cross-linking of IREM-1 also reversed the BAFF-mediated inhibition of phagocytosis. In order to demonstrate the role of ITIM in the IREM-1-mediated suppression of BAFF signalling, a decapeptide containing YADL (an ITIM in IREM-1) was fused with HIV-TAT(48-57) which was required for the internalization of the synthetic polypeptide (TAT-YADL). TAT-YADL, but not control peptides, recapitulated the effect of the anti-IREM-1 monoclonal antibody. These observations indicate that IREM-1 exerted its inhibitory effect on BAFF-medicated signalling through ITIM-mediated regulation of ERK activities in THP-1 cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-11147798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-11228151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-11742877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-12427767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-12975458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-15184070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-15549731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-15829264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-15886118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-16474316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-16487932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-17202342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-17647198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-18028878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-18097021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-18987138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-19290938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-19398961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-19440216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-19561101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-19841639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-19951174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20646006-20038601
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/B-Cell Activating Factor,
http://linkedlifedata.com/resource/pubmed/chemical/CD300LF protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 9,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/PTPN6 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatase...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/TNFSF13B protein, human
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1365-2249
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pubmed:author |
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pubmed:copyrightInfo |
© 2010 British Society for Immunology.
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pubmed:issnType |
Electronic
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pubmed:volume |
161
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
504-11
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pubmed:dateRevised |
2011-9-13
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pubmed:meshHeading |
pubmed-meshheading:20646006-Amino Acid Sequence,
pubmed-meshheading:20646006-Antibodies, Monoclonal,
pubmed-meshheading:20646006-B-Cell Activating Factor,
pubmed-meshheading:20646006-Cell Line,
pubmed-meshheading:20646006-Endocytosis,
pubmed-meshheading:20646006-Enzyme Activation,
pubmed-meshheading:20646006-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:20646006-Flow Cytometry,
pubmed-meshheading:20646006-HEK293 Cells,
pubmed-meshheading:20646006-Humans,
pubmed-meshheading:20646006-Interleukin-8,
pubmed-meshheading:20646006-Macrophages,
pubmed-meshheading:20646006-Matrix Metalloproteinase 9,
pubmed-meshheading:20646006-Oligopeptides,
pubmed-meshheading:20646006-Phosphorylation,
pubmed-meshheading:20646006-Protein Tyrosine Phosphatase, Non-Receptor Type 6,
pubmed-meshheading:20646006-Receptors, Immunologic,
pubmed-meshheading:20646006-Signal Transduction,
pubmed-meshheading:20646006-Transfection,
pubmed-meshheading:20646006-U937 Cells
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pubmed:year |
2010
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