Linked Life Data

2060788

Source:http://linkedlifedata.com/resource/pubmed/id/2060788

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1991-8-5
pubmed:databankReference
pubmed:abstractText
The transcription and translation of the tellurite-resistance (TeR) genes of the HII incompatibility group plasmid, pHH1508a, were studied. The nucleotide (nt) sequence of the TeR region was determined and two possible open reading frames, tehA and tehB, were identified. The direction of transcription and translation of these genes was confirmed through the preparation of lacZ and phoA (encoding alkaline phosphatase) fusions. The transcription start point was identified in the sequence using RNA primer extension. The tehA gene codes for a 36-kDa polypeptide which is highly hydrophobic. The TehA protein appears to be located in the inner membrane of the bacterial cell since tehA fusions with both phoA and lacZ were obtained and expressed. The tehB gene codes for a 23-kDa polypeptide which appears to be relatively hydrophilic and is probably located in the cytoplasm. Both proteins were overproduced using a T7 RNA polymerase/promoter system. No nt or amino acid sequence homology could be found between this TeR determinant and the TeR genes from the IncHI-2 plasmid, pMER610, and the IncP alpha plasmid, RK2.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
101
pubmed:geneSymbol
lacZ, phoA, tehA, tehB
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Nucleotide sequence and overexpression of the tellurite-resistance determinant from the IncHII plasmid pHH1508a.
pubmed:affiliation
Department of Medical Microbiology, University of Alberta, Edmonton, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't