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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5985
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pubmed:dateCreated |
2010-6-18
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pubmed:databankReference |
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pubmed:abstractText |
In Drosophila, microRNAs (miRNAs) typically guide Argonaute1 to repress messenger RNA (mRNA), whereas small interfering RNAs (siRNAs) guide Argonaute2 to destroy viral and transposon RNA. Unlike siRNAs, miRNAs rarely form extensive numbers of base pairs to the mRNAs they regulate. We find that extensive complementarity between a target RNA and an Argonaute1-bound miRNA triggers miRNA tailing and 3'-to-5' trimming. In flies, Argonaute2-bound small RNAs--but not those bound to Argonaute1--bear a 2'-O-methyl group at their 3' ends. This modification blocks target-directed small RNA remodeling: In flies lacking Hen1, the enzyme that adds the 2'-O-methyl group, Argonaute2-associated siRNAs are tailed and trimmed. Target complementarity also affects small RNA stability in human cells. These results provide an explanation for the partial complementarity between animal miRNAs and their targets.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AGO1 protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/AGO2 protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/Argonaute Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Eukaryotic Initiation Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hen1 protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/Let-7 microRNA, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/Methyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/MicroRNAs,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Caps,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Induced Silencing Complex,
http://linkedlifedata.com/resource/pubmed/chemical/bantam microRNA, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/enhanced green fluorescent protein
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1095-9203
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
18
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pubmed:volume |
328
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1534-9
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:20558712-Animals,
pubmed-meshheading:20558712-Argonaute Proteins,
pubmed-meshheading:20558712-Base Pairing,
pubmed-meshheading:20558712-Cell Line,
pubmed-meshheading:20558712-Drosophila Proteins,
pubmed-meshheading:20558712-Drosophila melanogaster,
pubmed-meshheading:20558712-Eukaryotic Initiation Factors,
pubmed-meshheading:20558712-Green Fluorescent Proteins,
pubmed-meshheading:20558712-Humans,
pubmed-meshheading:20558712-Methylation,
pubmed-meshheading:20558712-Methyltransferases,
pubmed-meshheading:20558712-MicroRNAs,
pubmed-meshheading:20558712-Models, Biological,
pubmed-meshheading:20558712-RNA, Complementary,
pubmed-meshheading:20558712-RNA, Messenger,
pubmed-meshheading:20558712-RNA, Small Interfering,
pubmed-meshheading:20558712-RNA Caps,
pubmed-meshheading:20558712-RNA Stability,
pubmed-meshheading:20558712-RNA-Induced Silencing Complex
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pubmed:year |
2010
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pubmed:articleTitle |
Target RNA-directed trimming and tailing of small silencing RNAs.
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pubmed:affiliation |
Howard Hughes Medical Institute and Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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