Linked Life Data

20437167

Source:http://linkedlifedata.com/resource/pubmed/id/20437167

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2010-5-18
pubmed:abstractText
In many bacterial species, the translational GTPase TypA acts as a global stress- and virulence regulator and also mediates resistance to the antimicrobial peptide BPI. On the chromosome of M. tuberculosis, typA is located next to narGHJI, which plays a role in adaptation of the pathogen to various environmental conditions. Here, we show that Mycobacterium tuberculosis is sensitive to P2, a derivative of BPI. Using a typA mutant of M. tuberculosis, we found this phenotype to be independent of TypA. We further tested typA expression in M. tuberculosis under defined stress conditions, such as oxygen- and nutrient depletion, low pH, heat shock, antibiotic stress and the presence of P2, and found that typA expression remains unaffected by any of these conditions. Analysis of growth and whole-genome expression revealed similar growth kinetics and gene expression profiles of the wild type and the mutant under normal growth conditions as well as under stress conditions. Our results suggest that in contrast to the findings in other bacteria, TypA does not act as a global stress- and virulence regulator in M. tuberculosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1432-072X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
192
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
499-504
pubmed:meshHeading
pubmed-meshheading:20437167-Adaptation, Physiological, pubmed-meshheading:20437167-Antimicrobial Cationic Peptides, pubmed-meshheading:20437167-Bacterial Proteins, pubmed-meshheading:20437167-Blood Proteins, pubmed-meshheading:20437167-GTP Phosphohydrolases, pubmed-meshheading:20437167-Gene Deletion, pubmed-meshheading:20437167-Gene Expression Profiling, pubmed-meshheading:20437167-Gene Expression Regulation, Bacterial, pubmed-meshheading:20437167-Heat-Shock Response, pubmed-meshheading:20437167-Microbial Viability, pubmed-meshheading:20437167-Mycobacterium tuberculosis, pubmed-meshheading:20437167-Peptide Fragments, pubmed-meshheading:20437167-Ribosomes, pubmed-meshheading:20437167-Stress, Physiological, pubmed-meshheading:20437167-Transcription, Genetic, pubmed-meshheading:20437167-Virulence
pubmed:year
2010
pubmed:articleTitle
Analysis of expression and regulatory functions of the ribosome-binding protein TypA in Mycobacterium tuberculosis under stress conditions.
pubmed:affiliation
Department of Medical Microbiology and Hospital Epidemiology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't