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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
2010-6-10
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pubmed:abstractText |
Coronavirus nonstructural proteins 1 to 3 are processed by one or two papain-like proteases (PLP1 and PLP2) at specific cleavage sites (CS1 to -3). Murine hepatitis virus (MHV) PLP2 and orthologs recognize and cleave at a position following a p4-Leu-X-Gly-Gly-p1 tetrapeptide, but it is unknown whether these residues are sufficient to result in processing by PLP2 at sites normally cleaved by PLP1. We demonstrate that exchange of CS1 and/or CS2 with the CS3 p4-p1 amino acids in engineered MHV mutants switches specificity from PLP1 to PLP2 at CS2, but not at CS1, and results in altered protein processing and virus replication. Thus, the p4-p1 residues are necessary for PLP2 processing but require a specific protein or cleavage site context for optimal PLP recognition and cleavage.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-10644337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-10708455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-10725411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-10823872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-10933699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-11431476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-11831690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-12368349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-12927536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-14752165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-15140993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-15479796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-15564471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-16227261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-16306591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-16358325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-16581910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-16971428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-17251282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-17855548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-19430490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-2526320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-2720781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-7539970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-8995606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20427532-9444982
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1098-5514
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6894-8
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pubmed:dateRevised |
2011-7-19
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pubmed:meshHeading |
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pubmed:year |
2010
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pubmed:articleTitle |
Exchange of the coronavirus replicase polyprotein cleavage sites alters protease specificity and processing.
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pubmed:affiliation |
Department of Pediatrics, Vanderbilt University Medical Center, 1161 21st Ave. S., Nashville, TN 37232-2581, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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