Linked Life Data

20334581

Source:http://linkedlifedata.com/resource/pubmed/id/20334581

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
2010-3-25
pubmed:abstractText
AMP-activated protein kinase (AMPK), a serine/threonine kinase activated upon energy depletion, stimulates energy production and limits energy utilization. It has previously been shown to enhance cellular glucose uptake through the GLUT family of facilitative glucose transporters. The present study explored the possibility that AMPK may regulate Na+-coupled glucose transport through SGLT1 (SLC5A1). To this end, SGLT1 was expressed in Xenopus oocytes with and without AMPK and electrogenic glucose transport determined by dual electrode voltage clamping experiments. In SGLT1-expressing oocytes but not in oocytes injected with water or expressing constitutively active (gammaR70Q)AMPK (alpha1beta1gamma1(R70Q)) alone, the addition of glucose to the extracellular bath generated a current (I(g)), which was half maximal (K(M)) at approximately 650 microM glucose concentration. Coexpression of (gammaR70Q)AMPK did not affect K(M) but significantly enhanced the maximal current (approximately 1.7 fold). Coexpression of wild type AMPK or the kinase dead (alphaK45R)AMPK mutant (alpha1(K45R)beta1gamma1) did not appreciably affect I(g). According to confocal microscopy and Western Blotting, AICAR (1 mM), phenformin (1 mM) and A-769662 (10 microM) enhanced the SGLT1 protein abundance in the cell membrane of Caco2 cells suggesting that AMPK activity may increase membrane translocation of SGLT1. These observations support a role for AMPK in the regulation of Na+-coupled glucose transport.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1464-5203
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
137-44
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Regulation of Na+-coupled glucose carrier SGLT1 by AMP-activated protein kinase.
pubmed:affiliation
Department of Physiology, University of Tübingen, Tübingen, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't