Linked Life Data

20155821

Source:http://linkedlifedata.com/resource/pubmed/id/20155821

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2010-4-8
pubmed:abstractText
The zebrafish has become a new model for adult neurogenesis, owing to its abundant neurogenic areas in most brain subdivisions. Radial glia-like cells, actively proliferating cells, and label-retaining progenitors have been described in these areas. In the telencephalon, this complexity is enhanced by an organization of the ventricular zone (VZ) in fast and slow-dividing domains, suggesting the existence of heterogeneous progenitor types. In this work, we studied the expression of various transgenic or immunocytochemical markers for glial cells (gfap:gfp, cyp19a1b:gfp, BLBP, and S100beta), progenitors (nestin:gfp and Sox2), and neuroblasts (PSA-NCAM) in cycling progenitors of the adult zebrafish telencephalon (identified by expression of proliferating cell nuclear antigen (PCNA), MCM5, or bromodeoxyuridine incorporation). We demonstrate the existence of distinct populations of dividing cells at the adult telencephalic VZ. Progenitors of the overall slow-cycling domains express high levels of Sox2 and nestin:gfp as well as all glial markers tested. In contrast, domains with an overall fast division rate are characterized by low or missing expression of glial markers. PCNA-positive cells in fast domains further display a morphology distinct from radial glia and co-express PSA-NCAM, suggesting that they are early neuronal precursors. In addition, the VZ contains cycling progenitors that express neither glial markers nor nestin:gfp, but are positive for Sox2 and PSA-NCAM, identifying them as committed neuroblasts. On the basis of the marker gene expression and distinct cell morphologies, we propose a classification for the dividing cell states at the zebrafish adult telencephalic VZ.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1098-1136
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
870-88
pubmed:meshHeading
pubmed-meshheading:20155821-Animals, pubmed-meshheading:20155821-Animals, Genetically Modified, pubmed-meshheading:20155821-Biological Markers, pubmed-meshheading:20155821-Cell Differentiation, pubmed-meshheading:20155821-Cell Division, pubmed-meshheading:20155821-Cell Proliferation, pubmed-meshheading:20155821-Intermediate Filament Proteins, pubmed-meshheading:20155821-Lateral Ventricles, pubmed-meshheading:20155821-Nerve Tissue Proteins, pubmed-meshheading:20155821-Neural Cell Adhesion Molecule L1, pubmed-meshheading:20155821-Neurogenesis, pubmed-meshheading:20155821-Neuroglia, pubmed-meshheading:20155821-Neuronal Plasticity, pubmed-meshheading:20155821-Neurons, pubmed-meshheading:20155821-SOX Transcription Factors, pubmed-meshheading:20155821-Sialic Acids, pubmed-meshheading:20155821-Stem Cells, pubmed-meshheading:20155821-Telencephalon, pubmed-meshheading:20155821-Zebrafish, pubmed-meshheading:20155821-Zebrafish Proteins
pubmed:year
2010
pubmed:articleTitle
Heterogeneity in progenitor cell subtypes in the ventricular zone of the zebrafish adult telencephalon.
pubmed:affiliation
Institute for Toxicology and Genetics, Forschungszentrum Karlsruhe in the Helmholtz Association, Karlsruhe Institute of Technology, Postfach 3640, 76021 Karlsruhe, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't