Source:http://linkedlifedata.com/resource/pubmed/id/20118359
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2010-3-5
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pubmed:abstractText |
The Ibis T5000 is a novel diagnostic platform that couples PCR and mass spectrometry. In this study, we developed an assay that can identify all known pathogenic Vibrio species and field-tested it using natural water samples from both freshwater lakes and the Georgian coastal zone of the Black Sea. Of the 278 total water samples screened, 9 different Vibrio species were detected, 114 (41%) samples were positive for V. cholerae, and 5 (0.8%) samples were positive for the cholera toxin A gene (ctxA). All ctxA-positive samples were from two freshwater lakes, and no ctxA-positive samples from any of the Black Sea sites were detected.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1098-5336
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pubmed:author |
pubmed-author:BaldwinCarsonC,
pubmed-author:BlynLawrence BLB,
pubmed-author:ColwellRita RRR,
pubmed-author:GrimChristopherC,
pubmed-author:HallThomas ATA,
pubmed-author:HarpinVanessaV,
pubmed-author:HuqAnwarA,
pubmed-author:JaianiEkaterinaE,
pubmed-author:JanelidzeNinoN,
pubmed-author:KokashviliTamarT,
pubmed-author:LiFengF,
pubmed-author:MatthewsHeatherH,
pubmed-author:MeltonRachaelR,
pubmed-author:SampathRangarajanR,
pubmed-author:TediashviliMarinaM,
pubmed-author:WhitehouseChris ACA
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pubmed:issnType |
Electronic
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pubmed:volume |
76
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1996-2001
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pubmed:dateRevised |
2010-9-2
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pubmed:meshHeading |
pubmed-meshheading:20118359-Bacteriological Techniques,
pubmed-meshheading:20118359-Cholera Toxin,
pubmed-meshheading:20118359-DNA, Bacterial,
pubmed-meshheading:20118359-Fresh Water,
pubmed-meshheading:20118359-Georgia,
pubmed-meshheading:20118359-Polymerase Chain Reaction,
pubmed-meshheading:20118359-Seawater,
pubmed-meshheading:20118359-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:20118359-Vibrio
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pubmed:year |
2010
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pubmed:articleTitle |
Identification of pathogenic Vibrio species by multilocus PCR-electrospray ionization mass spectrometry and its application to aquatic environments of the former soviet republic of Georgia.
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pubmed:affiliation |
U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MA, USA. chris.whitehouse@us.army.mil
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Evaluation Studies,
Research Support, N.I.H., Extramural
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